Single-Cell Spatial Analysis Reveals Early Transcriptional Changes in Pancreatic Tumorigenesis
Despite being first described in 1905, pancreatic intraepithelial neoplasias (PanIN) are still poorly understood. In particular, the mechanisms involved in the progression of PanIN lesions into invasive pancreatic adenocarcinoma remain unclear.
One of the challenges with molecular and cellular profiling is the formalin-fixation and paraffin-embedding of PanIN samples, a process that damages valuable transcriptomic information.
In this webinar, Dr. Luciane Kagohara from Johns Hopkins Medicine will discuss a novel artificial intelligence method integrating imaging, spatial transcriptomics and single-cell RNA-seq data that has been used to characterize the cell state transitions in pancreatic cancer progression.
Attend this webinar to discover:
- The link between inflammatory signaling, cellular proliferation and PanIN progression
- How single-cell spatial transcriptomics profiling provides key information on the cellular transitions in cancer
- A new protocol for spatial transcriptomics profiling of PanIN specimen