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Application of Dynamic Light Scattering (DLS) to Protein Therapeutic Formulations: Principles, Measurements and Analysis - 3. DLS Deconvolution Algorithms

Dynamic light scattering (DLS) is an analytical technique used to measure the
particle size distribution of protein formulations across the oligomer and sub-micron
size ranges of approximately 1 nm to 1 μm. The popularity of DLS within the
biopharmaceutical industry is a consequence of its wide working size and extended
sample concentration ranges, as well as its low volume requirements. With that
said, the challenge that remains with the application of DLS to protein therapeutic
formulations is centered around data interpretation. In this four-part white paper
series, common issues and questions surrounding the principles, measurements and
analysis of DLS data are discussed in order to help minimize the time required for
and complexity of acquiring and interpreting DLS data that is critical throughout the
development process. In this third white paper of the series, we cover the basic types
of DLS deconvolution algorithms used to extract the intensity weighted particle size
distribution from the measured correlogram.