Techniques for Oligonucleotide Analysis
Oligonucleotides (short fragments of DNA or RNA) are an important resource for diagnostics and therapeutics. But in order for them to be clinically useful without adverse side effects, their synthesis and characterization must be rigorous.
Despite the increasing use of oligonucleotides in biomedical settings, their purification and characterization of their structural properties remain challenging.
This article presents current techniques in oligonucleotide production and characterization, highlighting recent advancements that enhance the efficiency of these methods
- Common impurities introduced during oligonucleotide synthesis.
- Oligonucleotide purification and mass characterization techniques.
- Techniques to resolve oligonucleotide structures.