Genotyping of Human Reference DNA Samples with Pharmacologically Important Single Nucleotide Polymorphisms using KASP™ Genotyping Chemistry on the Nexar® SystemApplication Note
Five pharmacologically important SNPs were analyzed with a miniaturized PCR-based SNP genotyping method using KASP™ genotyping reagents and the Douglas Scientific® Nexar®. The Nexar along with Array Tape® significantly reduces reaction volumes, hands-on effort and over-all cost of SNP genotyping, leading to a reliable and economical alternative to traditional PCR-based SNP genotyping techniques.READ MORE
Using the µEncapsulator 1 to Capture Single Cells in Droplets with BeadsApplication Note
In order to simplify high throughput single cell methods, Dolomite has developed the µEncapsulator 1 System, a compact module that simply and securely holds a droplet generating chip, convenient sample reservoirs, and an output reservoirREAD MORE
Evaluation of the IntelliQube® for Gene Expression Analysis using a One-Step RT-PCR WorkflowApplication Note
A recent study demonstrates the ability of the IntelliQube® and associated Array Tape®, to process RNA samples using a one-step RT-PCR workflow, opening the door to a variety of RNA screening applications including gene expression or viral detection and quantification.READ MORE
Evaluation of a New Accurate, Automated and Cost-effective Approach for the Analysis of Pharmacologically Important Copy Number VariationApplication Note
The IntelliQube® from Douglas Scientific® is designed to address the need for accurate and reliable results for studies such as CNV determination.READ MORE
IntelliQube® Real-Time Quantitative PCR PerformanceApplication Note
The IntelliQube performs at an industry leading level, facilitating outstanding qPCR results with Array Tape® and the added benefits of inline automation. Liquid handling, thermal cycling, and detection systems within the IntelliQube enables users to achieve efficient and economical high throughput sample processing in Array Tape without compromising important parameters of qPCR performance.READ MORE
3D Cell Culture: mimicking real tissueApplication Note
Tecan Group Ltd.
Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory.
Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
A Fluorescence Based Assay of the Epigenetic Enzyme Histonedeacetylase 1 (HDAC1)Application Note
Post-translational histone modifications like acetylation play a pivotal role in the epigenetic regulation of transcription. Catalyzing the latter reaction HDACs affect various cellular processes especially cancerogenesis. Although the mechanism of starting cancerogenesis by epigenetic events is not clearly explained inhibition of HDACs has highlighted as a viable principle in cancer therapy.READ MORE
Measurement of Proteasome Inhibition in Live Cells in Molecular Devices Microplate FluorometersApplication Note
The proteasome is a massive protein complex which can recognize and bind targeted proteins, and inject them into the central core where the proteins are successively degraded into short peptides. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.READ MORE