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Improving Complex Phosphopeptide Characterization

Credit: iStock

Phosphorylation of the casein proteins in milk is required to facilitate the adsorption of these nutrients in the gut of a nursing infant.

Characterizing phosphorylation sites on casein proteins is inherently difficult due to the presence of peptides with highly variable levels of phosphorylation. However, using a hybrid electron-activated dissociation (EAD) and collision-induced dissociation (CID) fragmentation approach can improve the sequence coverage for casein peptides and post-translational modification (PTM) characterization.

This app note highlights the use of EAD/CID to enhance the fragmentation of peptides from casein tryptic digests.

Download this app note to discover a system that can:

  • Elucidate complex peptide sequences and correctly assign PTMs
  • Generate information-rich MS/MS spectra with excellent sequence coverage
  • Quantify and rapidly monitor the different phosphopeptide variants in casein extracts
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