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Quantifying T Cell Response in 3D Tumor Spheroids Using Advanced Flow Cytometry Workflows

Discovery of novel immunotherapies that specifically target and enhance the T cell response against cancer is a rapidly expanding research area. Robust and relevant in vitro models for evaluation of these immunotherapies is essential throughout their development. In the past, these models have often relied on the use of suspension cells which has limited studies to liquid tumors, such as blood cancers. Others have used 2D cell monolayers to explore drug effects on solid tumors, but these models lack many of the key features of the in vivo tumor microenvironment.

Download this app note to learn more about a platform that can be used to: 

  • Investigate solid tumor therapies
  • Analyze and compare infiltrated immune cell populations
  • Monitor fluctuations in target cell viability, marker expression and cytokine release