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Simple Steps To Publication-Quality Western Blots

The use of western blot protein detection is a simple technique, that unlike other antibody-based detection methods, provides information on protein size, which has fed its growth in popularity since being introduced. Recently however, there have been concerns over the reproducibility and quality of western blotting data. Many publications have revised their instructions for submitting western blot data to reflect best practice.

Download this app note to discover tips on avoiding some of the pitfalls of these new stringent guidelines including:

  • Image collection and presentation
  • Validating primary antibodies
  • Improving band and background resolution