Introduction to Metabolomics Analysis
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Date:
Tuesday 20 - Friday 23 March 2018Venue:
European Bioinformatics Institute (EMBL-EBI) - Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SD, United KingdomApplication opens:
Monday 18 September 2017Application deadline:
Saturday 13 January 2018Participation:
Open application with selectionContact:
Emily ReesRegistration fee:
£300Registration closed
Overview
This course will provide an introduction to metabolomics data analysis using publicly available software and tools. Participants will become familiar with the current state of experimental design, data standards, analysis and sharing in metabolomics, particuarly through using the EMBL-EBI's MetaboLights repository. There will be a large practical component, where participants will learn through hands-on tutorials to use a workflow-based approach and compute infrastructure for data analysis and data submission, under the guidance of the lecturers and teaching assistants.
Audience
This course is aimed at researchers who are new to the field of metabolomics and wish to learn the basics. Perhaps you normally work in a different field and have had the opportunity to send off some samples for metabolite profiling but are unsure about how to process your results - this course is for you. We will primarily focus on a basic introduction to metabolomics with a worked example using a predesigned LC-MS analysis workflow. The course assumes little prior knowledge of using bioinformatics tools.
Syllabus, tools and resources
During this course you will learn about:
- Basic metabolomics
- Metabolomics resources: MetaboLights, Phenomenal, MetaboFlow
- Metabolomics tools made available through standardized, customisable workflows
Outcomes
After this course you should be able to:
- Discuss basic analytical methods in metabolomics, and factors that contribute to designing a successful metabolomics study
- Use standardized workflows to perform basic metabolomics analysis
- Access metabolomics databases
- Discuss the issues of data sharing and data standards in metabolomics
Programme
Time | Topic | Trainer |
---|---|---|
Day 1 - Tue 20th March | ||
12:00 | Registration and lunch | |
13:00 - 13:30 | An introduction to the EMBL-EBI | Alexandra Holinski |
13:30 - 15:00 | An introduction to Metabolomics | Keeva Cochrane, Kenneth Haug, Venkata Chandrasekhar Nainala |
15:00 - 15:30 | Tea/coffee break | |
15:30 - 16:30 | An introduction to Metabolomics Resources & Data Sharing | Keeva Cochrane, Kenneth Haug, Venkata Chandrasekhar Nainala |
16:30 - 17:00 | Interactive session | Keeva Cochrane, Kenneth Haug, Venkata Chandrasekhar Nainala |
18:00 | Evening meal at Wellcome Genome Campus Conference Centre | |
Day 2 - Wed 21th March | ||
09:30 - 10:00 | An introduction to MetaboLights | Keeva Cochrane, Kenneth Haug, Venkata Chandrasekhar Nainala |
10:00 - 10:30 | Creating a MetaboLights workspace (hands-on session) | Keeva Cochrane, Kenneth Haug, Venkata Chandrasekhar Nainala |
10:30 - 11:00 | Tea/coffee break | |
11:00 - 12:00 | Basic experimental design | Ralf Weber / Nicola Gray |
12:00 - 13:00 | An introduction to analytical platforms; an overview | Ralf Weber / Nicola Gray |
13:00 - 14:00 | Lunch | |
14:00 - 14:30 | An introdcution to analytcial platforms; LC-MS | Ralf Weber / Nicola Gray |
14:30 - 15:30 | What is a workflow? Basics of metabolomics analysis | Ralf Weber / Nicola Gray |
15:30 - 16:00 | Tea/coffee break | |
16:00 - 17:30 | Part 1 Hands-on standardized LC-MS workflow: working with raw data | Ralf Weber / Nicola Gray |
17.30 - 18.30 | Networking/Posters | Ralf Weber / Nicola Gray / Metabolomics team |
18:30 | Evening meal at Wellcome Genome Campus Conference Centre | |
Day 3 - Thu 22th March | ||
09:30 - 11:00 | Introduction to data interpretation & statistics | Etienne Thevenot |
11:00 - 11:30 | Tea/coffee break | |
11:30 - 13:00 | Part 2 Hands-on standardized LC-MS workflow: Data interpretation and statistical analysis | Etienne Thevenot |
13:00 - 14:00 | Lunch | |
14:00 - 15:30 | Introduction to metabolite identification methods | Ralf Weber |
15:30 - 16:00 | Tea/coffee break | |
16:00 - 18:00 | Part 3 Hands-on standardized LC-MS workflow: Metabolite identification | Ralf Weber |
18.30 | Evening meal at Red Lion, Hinxton | |
Day 4 - Fri 23th March | ||
09:30 - 10:30 | Introduction to network & pathway analysis | Clement Frainay |
10:30 - 11:00 | Part 4 Hands-on standardized LC-MS workflow: Introduction to network & pathway analysis | Clement Frainay |
11:00 - 11:30 | Tea/coffee break | |
11:30 - 12:30 | Part 4 cont. Hands-on standardized LC-MS workflow: Introduction to network & pathway analysis | Clement Frainay |
12:30 - 13:00 | MetaboLights | Keeva Cochrane, Kenneth Haug, Venkata Chandrasekhar Nainala |
13:00 - 13:15 | Wrap up & Course feedback | Alexandra Holinski |
13:15 - 14:00 | Lunch | |
14:00 | Coach departs to Cambridge Train Station |