DIA vs DDA in Proteomics
Infographic Sep 03, 2019
Over recent years, liquid chromatography coupled to tandem mass-spectrometry (LC-MS/MS) has become the MS technology of choice for the high-throughput characterization of proteins and proteomes.
There are two modes of acquisition that can be used in LC-MS/MS experiments: the more traditional data dependent acquisition (DDA) mode, and the recently developed data independent acquisition (DIA) approaches, such as SWATH.
Download this infographic to gain an understanding of:
- The processes involved in an experiment using a DIA approach
- How DIA produces a higher number of quantified proteins in a shorter analysis period than DDA
For proteomics researchers, work is almost impossible without mass spectrometry (MS). Neuroscience, however, has largely got by without dipping its toe into the pool of analytical chemistry. With the advent of faster, smarter MS tools, those days may be numbered, as MS-based techniques become an invaluable resource for studying the brain.READ MORE