The paper, entitled 'Discovery pipeline for epigenetically deregulated miRNAs in cancer: integration of primary miRNA transcription' is available online through BioMed Centrals Genomics journal.
Cancer is commonly associated with widespread disruption of DNA methylation, chromatin
modification and miRNA expression. In this study, we established a robust discovery pipeline to
identify epigenetically deregulated miRNAs in cancer.
Using an integrative approach that combines primary transcription, genome-wide DNA methylation
and H3K9Ac marks with microRNA (miRNA) expression, we identified miRNA genes that were
epigenetically modified in cancer. We find miR-205, miR-21, and miR-196b to be epigenetically
repressed, and miR-615 epigenetically activated in prostate cancer cells.
We show that detecting changes in primary miRNA transcription levels is a valuable method for
detection of local epigenetic modifications that are associated with changes in mature miRNA