Mutations Previously Linked to Breast Cancer Found Not To Increase Risk of Disease
Complete the form below to unlock access to ALL audio articles.
Several genetic mutations previously linked to breast cancer and included on commercial genetic tests, including direct-to-consumer tests, were found not to increase a woman’s risk of disease, according to a population study of more than 64,000 women published online today in the New England Journal of Medicine from several institutions, including Penn Medicine. The findings show that risks associated with mutations for women in the general population are often lower than previous estimates, and, importantly, provide new insights informing the debate over whom should be recommended for genetic testing.
Penn Medicine authors on the paper — which analyzed data from the CARRIERS study, or “CAnceR RIsk Estimates Related to Susceptibility”— include Katherine L. Nathanson, MD, deputy director of the Abramson Cancer Center and the Pearl Basser Professor for BRCA-Related Research in the Perelman School of Medicine at the University of Pennsylvania, and Susan M. Domchek, MD, executive director of the Basser Center for BRCA at the Abramson Cancer Center. Fergus J. Couch, PhD, of the Mayo Clinic, serves as the senior author.
According to past estimates, seven to 10 percent of women with breast cancer carry pathogenic variants in genes associated with an increased risk. However, that statistic is based largely on studies of high-risk women, including those with a family history or a young age of cancer diagnosis.
This study—the first to look at a large group of women of different ages from the general population—suggests that the frequency of pathogenic variants in genes associated with breast cancer risk among women in the general population is five percent. Further, of the 28 breast cancer genes studied, only 12 had clear evidence of associated cancer risk.
“These findings have important implications for the clinical management of patients,” Nathanson said. “This study has a more accurate risk estimate for the overall breast cancer population, as opposed to selected high-risk patients, that we believe will inform ongoing and future discussions around genetic testing for women and other risk management strategies.”
Today, recommendations for genetic screening vary, from testing all patients for genes associated with breast cancer to testing affected and unaffected women based on risk stratification. Many commercially available hereditary genetic tests also include a slew of genes that now appear, based off these findings, to not increase risk, which has the potential to lead to the delivery of misinformation, as well as affect treatment decisions.
In the study, pathogenic variants were found through multiplex gene sequencing in 12 established genes among 32,247 women with breast cancer and 32,544 women without breast cancer, including: ATM, BARD1, BRCA1, BRCA2, CDH1, CHEK2, NF1, PALB2, PTEN, RAD51C, RAD51D, and TP53.
Those pathogenic variants were detected in 5.01 percent of breast cancer cases and 1.63 percent cases in the control group.
Several candidate genes previously associated with increased breast cancer risk and often present on commercial testing panels, including NBN, BRIP1 and RECQL, showed no associations with breast cancer risk. In particular, the finding that the NBN c.657_661del5 Slavic founder mutation was not associated with an increased risk of breast cancer suggests that increased screening of women with NBN pathogenic variants recommended by management guidelines may need to be re-evaluated, the authors said.
Other mutations commonly linked to breast cancer that also showed no association with risk include BLM, CDKN2A, ERCC3, FANCC, FANCM, MRE11A, MSH2, MSH6, RAD50, RINT1, SLX4, and XRCC2.
In all, the CARRIERS consortium included patients from 17 breast cancer case-control studies.
Notably, this new study demonstrated that certain subgroups of breast cancer patients have substantially increased risk of having high penetrance, clinically actionable pathogenic variants. For example, BRCA1, BRCA2, and PALB2 pathogenic variants were observed in 8.13 percent in patients with triple negative breast cancer compared to 1.84 percent in ER-positive patients.
In addition, pathogenic variants in BARD1, RAD51C, and RAD51D showed weak associations with overall risk but were associated with moderate risks of ER-negative breast cancer, a disease that disproportionally affects Black women.
“Thus, risk stratification of women with breast cancer in the general population based on features such as tumor markers is an important method for identifying women at the highest risk of having a mutation, especially among underserved, minority populations,” the authors wrote.
The most common genes with pathogenic variants among the study group were CHEK2 and ATM, while pathogenic variants in BRCA1 and BRCA2 were found in 0.35 percent of the study participants.
Domchek and Nathanson both sit on the executive committee for the CARRIERS study.
“This multi-institutional, collaborative study shows us a clearer picture of risk and genetic drivers for women in the general population who don’t fall under the high-risk category,” Domchek said. “As discussions continue regarding the role of population screening, the CARRIERS data support careful gene selection.”
Reference: Hu C, Hart SN, Gnanaolivu R, et al. A population-based study of genes previously implicated in breast cancer. New Eng. J. Med. 2021 doi:10.1056/NEJMoa2005936
This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.