Studying Adhesion Forces Between Cancer Cells
News Feb 18, 2014
JPK Instruments reports on the current cancer research project of Dr Florian T Ludwig at the Institute of Physiology II, University Hospital Münster, who uses a CellHesion® 200 to measure the adhesive forces between tumor cells.
The Institute of Physiology II is part of the Medical Faculty of the Westfälische Wilhelms-Universität Münster. It brings together the areas of physiology, cell migration and ion transporters through the study of properties of cells on the nanoscale.
Dr Florian T Ludwig is a member of the team of Professor Albrecht Schwab and Dr Christian Stock. These researchers are interested in how ion transport across the plasma membrane affects tumor cell motility and, thus, metastasis. Occurring prior to migration and invasion, one early step of the so-called metastatic cascade is the detachment of single tumor cells or cell clusters from the primary neoplasm. This process is accompanied by a controlled loss of cell-cell adhesions caused by the release of bonds between adhesion molecules such as cadherins. Hence, the adhesion force between tumor cells might predict the tumor's malignancy. Tumor cells that exhibit low cell-cell adhesion forces would represent a highly metastatic phenotype since they detach more easily.
With the help of Mike Wälte from the group of the Head of the Institute, Professor Hans Oberleithner and Dr Hermann Schillers, Dr. Ludwig measures cell-cell adhesion forces in tumor cells. This is based on the group's distinguished expertise of nearly two decades' experience in atomic force microscopy, AFM.
For the experiments, a single tumor cell is attached to the cantilever of a JPK CellHesion® 200 system using a bionic cell adhesive. A second, adherent tumor cell is then approached with the cantilever until the two cells come into contact. After a defined contact time, the cantilever is retracted until the two cells are separated. The strength of the cell-cell bonds that formed while the cells were in contact is estimated by recording the force necessary to separate the cells. Here, the key feature of the CellHesion® 200 system is its ability of the cantilever sensor lifting system to travel more than 110 µm giving a clear benefit for the researchers whose aim is to separate two tumor cells both gently, reproducibly and efficiently without damage. With the technique described here, cell-cell adhesion forces can be quantified.
New Microscope Captures Detailed 3-D Movies of Cells Deep Within Living SystemsNews
Merging lattice light sheet microscopy with adaptive optics reveals the most detailed picture yet of subcellular dynamics in multicellular organisms.READ MORE
The End of the Breathalyzer?News
Engineers have developed a miniature, ultra-low power injectable biosensor that could be used for continuous, long-term alcohol monitoring. The chip is small enough to be implanted in the body just beneath the surface of the skin and is powered wirelessly by a wearable device, such as a smartwatch or patch.READ MORE
Reversing an Unstoppable Cancer Cascade with ProteomicsNews
Mutations in genes that produce RAS proteins turn a normally benign process, essential for cellular growth, into a cancer stimulant that is currently undruggable. Now, cutting-edge protein analysis may help treat cancers caused by these mutations.READ MORE
Comments | 0 ADD COMMENT
World Congress on Advanced Biomaterials and Tissue Engineering
Jun 20 - Jun 21, 2018
29th International Conference on Public Mental Health and Neuroscience
Jul 16 - Jul 18, 2018