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A Feeder-Free Method To Generate Functional Cytotoxic T Cells In Vitro

A 3D image of a cell surface interacting with T cells
Credit: iStock

Human induced pluripotent stem cells (iPSCs) have emerged as a powerful tool for generating genetically defined T cells for therapeutic use.


Conventional methods for T cell production often rely on feeder cell systems, which present reproducibility and safety challenges for clinical translation.


This poster presents a feeder-free protocol that enables scalable differentiation of iPSCs into CD8+ single-positive cytotoxic T cells, offering a robust platform for immunotherapy development.


Download this poster to discover:

  • A clinically relevant approach to generating CD8+ T cells from iPSCs
  • A feeder-free differentiation protocol that ensures consistency and scalability
  • How to achieve accurate T cell lineage commitment and maturation in vitro
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