Combining Imaging and OMICs: MALDI Guided SpatialOMx® Probes Proteomic Mass Profiles of Breast Cancer Tumor Subpopulations
Poster Apr 02, 2020
Janina Oetjen, Romano Hebeler, Frédéric Dewez, Corinna Henkel, Benjamin Balluff, Ron Heeren
While MALDI Imaging provides a direct link between tissue pathology and molecular expression, it lacks the in-depth molecular characterization attributed to 4D-OMICS strategies. SpatialOMx, an integration of MALDI Imaging with 4D-OMICs, yields a more comprehensive understanding of biochemical activity within the tissue microenvironment.
Download this poster to discover how the SpatialOMx workflow using timsTOF fleX provides detailed in-situ characterization of bioactivity across different tumor cell subpopulations by:
- Automated segmentation of tissue microenvironment based on label-free mapping of molecular expression
- Identification and location of distinctive tissue subtypes for laser microdissection and targeted OMICS analysis
- Performing in-depth 4D-proteomics analysis of excised material using PASEF
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License
OTHER POSTERS
When there is a need to quickly analyze samples using a number of different PCR assays, it is likely that optimal conditions for each assay will not be the same. First, different assays often will require different annealing temperatures for their primers. In addition, amplicons may be designed to be of different lengths and therefore require varying durations of the extension step.
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