Detection of 17 Targets in a Single PCR Tube by a Novel MeltPlex® Probe System Combining Melting Curves and Taqman Probes
Poster Sep 05, 2018
by Søren M Echwald.
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).
MeltPlex®utilizes a system of labelled Taqman-type probes allowing each to be read out by subsequent melting curve analysis. 5 or more probes can be analyzed per fluorophore channel. By utilizing melting curve readout of modified probes –one for each target -rather than the only amplicons, the system adds an extra level of specificity to melting curve analysis. Reaction and melting analysis is performed without the need to re-open PCR reaction tubes.
Detection reaction chemistry is similar to TaqMan and MeltPlex® probes can easily be designed using existing TaqMan target sequences
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE