A Life Sciences Renaissance: 125th Peer-Reviewed Publication Enabled By xCELLigence System
Product News Mar 26, 2011
To date, more than 125 peer-reviewed studies using Roche’s xCELLigence System have been published, spanning a diverse group of applications, including cell invasion and migration of cancer cells, receptor activity assays, compound induced cytotoxicity, antibody-dependent cellular cytotoxicity, virus-mediated cytopathogenicity and others.
The fields of research included toxicology, drug discovery, receptor biology, cancer research, inflammation, neurobiology, among others and are complemented by numerous review articles on the current uses and future potential of the xCELLigence System.
The 125th study, entitled “Differential roles of Src in transforming growth factor-β regulation of growth arrest, epithelial-to-mesenchymal transition and cell migration in pancreatic ductal adenocarcinoma cells,” was published in March 2011 in the International Journal of Oncology.
The authors Hendrik Ungefroren, Susanne Sebens, Stephanie Groth, Frank Gieseler and Fred Fändrich explored the potential role of the non-receptor tyrosine kinase Src in TGF-β1-mediated cellular responses using RNA and dominant-negative interference to block Src expression and function, respectively. For real-time measurement of cell migration, they used the xCELLigence RTCA DP Instrument from Roche.
The first xCELLigence System, the RTCA SP Instrument, was launched in mid-2008. The speed with which researchers using the xCELLigence System have been able to publish their discoveries demonstrates the high-quality of this analysis system, its ease of use and the reliability of the results.
“The xCELLigence System enables researchers to tackle innovative research, access diverse applications – and publish faster. Reaching 125 peer-reviewed publications so quickly is a testament to the widespread field of applications for which the system has been adopted,” explained Ruedi Stoffel, Life Cycle Leader Cellular Analysis at Roche Applied Science. Across all fields of cell science we have seen a fast growing number of papers in the last two years.”
The xCELLigence System monitors cellular events in real-time without the incorporation of labels. The system measures electrical impedance across interdigitated micro-electrodes integrated on the bottom of a tissue culture E-Plate.
The impedance measurement provides quantitative information about the biological status of the cells, including cell number, viability and morphology. A wide range of cell-based assays for both high-throughput screening and research laboratory environments can be performed on the xCELLigence System.