The Naica System for Crystal Digital PCR™
Stilla Technologies is a Paris-based European Biotechnology company that focuses on accelerating the development of next-generation genetic tests by providing an innovative instrumentation for digital PCR (dPCR).
In 2016, Stilla Technologies launched the Naica System, the first and only digital PCR solution to offer 3-color multiplexing. By encapsulating all steps for digital PCR in a single chip, the Naica System leverages the key assets of Crystal Digital PCR in a compact, easy-to-use, fast and reliable digital PCR solution.
Crystal Digital PCR is Stilla’s next-generation technology for absolute quantification of nucleic acids. Using cutting-edge microfluidic innovations, this technology integrates the Digital PCR process in a single consumable: The Sapphire chips.
Figure 1: Sapphire Chip
The sample is first flowed through a network of microchannels and partitioned into a large 2D array of 30,000 individual droplets, also called a droplet crystal. PCR is then performed on-chip and the crystal is imaged to reveal the droplets that contain amplified targets. The last step consists in counting the number of these positive droplets to precisely extract the absolute quantity of nucleic acids.
The Naica System Workflow:
Figure 2: Naica System Workflow
With Crystal Digital PCR, the combination of powerful image analysis and intuitive visual inspection offers an unmatched level of confidence in the digital PCR measurement, yielding data you can truly trust.
With the Naica System, you can use both hydrolysis probes and EvaGreen chemistries. You can perform PCR as well as One-step RT-PCR. You can therefore use both DNA or RNA as template.
In brief, the Naica System is:
An easy-to-use and integrated solution
A single consumable per experiment
Two instruments for the entire workflow
The fastest time-to-result on the market
Digital PCR results in 2h30, including thermocycling
Less than 5 min hands-on time
Reliable multiplex digital PCR
A unique 3-color detection
User-friendly analysis software
Applications
Crystal Digital™PCR for Cancer Research
Oncology is the key area of Digital PCR in which the technology is a major asset for the identification of DNA mutations (e.g EGFR), patients monitoring, gene amplification detection, CTC characterization, long non-coding RNA detection, circulating nucleic acids and tumor cells absolute quantification through liquid biopsies etc.
Other applications: Infectious Diseases, Prenatal testing, Organ transplant, Environment, Diabetes, Obesity, Food testing ect.
Social Media link:
- Linkedin : https://www.linkedin.com/company/stilla-technologies/
- Twitter : @StillaTech
- Youtube : https://www.youtube.com/channel/UCaPGZfNGCwaHCKUb_DBhkxQ
- Labtube: https://www.labtube.tv/channel/stillatech
