Gas Chromatography – Multimedia

High Resolution GC-TOFMS and GC×GC-TOFMS are useful for a non-target comprehensive characterization of petroleum through a petroleomic approach, and can also provide targeted analysis of chemical markers.

High Resolution GC-TOFMS analysis with high resolution and high mass accuracy is crucial for identification of crude oil and asphaltene pyrolysis products by unequivocal chemical formula assignment, with enhancement of sensitivity and selectivity.

Proper sample preparation is critical for the analysis of complex samples. GC-HRT provides high quality, accurate mass data for:
-Spectral similarity searches (NIST, Wiley, etc.)
-Formula determination (fragment, molecular, and adduct ions)

Analyses of the M. truncatula extracts were performed, and data processing focused on qualitative analyses. This provided the opportunity for known analyte verification and unknown identification in a meaningful matrix and the demonstration of the key attributes and benefits of GC-HRT.

High resolution GC-TOFMS on the Pegasus GC-HRT allowed for quick and easy non-targeted screening of extractables from a common consumer product: the ubiquitous cell phone case. Most components could be identified with high similarity scores against commercial libraries. However, even when faced with low similarity scores against commercial libraries, such as in the case of Biphenol AF, the ability to rely on high mass accuracy allowed for confident identification of components.

GC-HRT methods utilizing hydrogen carrier gas were developed for analysis of representative specialty chemical, forensic, and metabolomic markets.

• To demonstrate a fully automated workflow for both liquid injection and headspace for GC and GC/MS.
• Automatically optimize analysis conditions and derivatization parameters.
• Automate standard preparation and derivatization procedures.
• Reduce analysts time involved in the analysis.
• Compare manual versus automated preparation of standards.

This poster has demonstrated GC-TOFMS instrumentation applied to characterize beer-related samples. The ability to isolate and identify individual analytes through chromatographic resolution, mathematical deconvolution, and mass spectral searching to library standards provides good insight to various stages of production.

Purpose: An overview of the results of GC amenable compounds is given for EU 2013/39/EU
Methods: For the volatile compounds headspace GCMS was used; for the semivolatiles liquid liquid
extraction was applied followed by GCMSMS detection.
Results: The detection limits are calculated by standard deviation and there are some linearity examples. Not all compounds are listed in this poster, but a full list is available.

We introduce a new, innovative injector which allows conservation of helium carrier gas. While preserving the analytical GC column flow with helium, it maintains the septum purge and the split flows with another inert gas like nitrogen - even during the analytical run. The analytical performances of the injector are in line with those of the common split/splitless injector module

Purpose: Exploring the possibility to perform a quantitative determination of dissolved gases in water in a reliable and accurate way. Methods: The quantification of dissolved gases is performed by building a calibration curve spiking blank water samples with increasing quantities of saturated water followed by headspace sampling and GC/ FID analysis. Results: The results show good correlation factors and limit of quantification lower than 10ppb for the four gas analyzed.