Latest Application Notes
Live Cell Biosensor Assay Used To Interrogate GPCRs - Comparative Analysis of Biosensor Variants
This application note compares the performance of the highest and lowest affinity variants using automated methods and the highly-sensitive bottom reading capabilities of an HTS microplate reader. Sensitive detection of the 22F basal signal allows for use of this variant under a broad range of conditions, providing an assay with both superior dynamic range and sensitivity.
Direct Visualisation, Sizing and Counting of Aggregation in Proteins
Characterising the state of aggregation in proteins is of paramount importance when trying to understand biopharmaceutical product stability and efficacy. Product quality, both in terms of biological activity and immunogenicity can be highly influenced by the state of protein aggregation.
Automated Growth Monitoring of Microaerophilic Organisms
Walkaway growth monitoring of Helicobacter pylori in the Infinite® 200 PRO reader with Gas Control Module (GCM™).
Rapid Screening of a Cell-based Assay for GLP-1 Receptor Using a Natural Product Library
Here we demonstrate the detection of GLP-1R binding through the incorporation of a homogeneous cell-based binding assay using HTRF Tag-lite® technology.
Analyzing Biological Samples
This technical note describes the capability of Tecan’s Infinite® 200 PRO multimode reader to regulate the CO2 partial pressure inside its measurement chamber.
Advanced TC™: A Novel Cell Culture Surface Improving the Cultivation and Differentiation of Embryonic Stem Cells
The importance of embryonic stem cells rests in their lack of specialisation. These basic cells are present in the earliest stages of developing embryos and are able to develop into virtually any type of cell and tissue in the body.
ESGRO-2i Medium: Inhibitor-Based Serum-free Medium for ES and iPS Cell Culture
Defined serum-free and feeder-free culture of mouse embryonic stem (mES) cells holds many advantages over the classical serum-containing feeder-dependent culture methods, ranging from decreased lot-to-lot variations to ease of culture.
PSVue®: Novel small molecule probes for fluorescent imaging of apoptosis and bacterial infections in vitro and in vivo
PSVue® offers a robust alternative to probes currently used by researchers to detect early-to-mid stage apoptosis, as well as bacterial infections.
Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System
Accurate measurement of the concentration of a biological product is important in bioreactor/fermenter monitoring, downstream processing and final product quality control. Currently, electrophoretic, chromatographic and immunochemical methods are the most commonly used. These three methods are currently used in combination to provide the desired sample information.
Dual-Glo Luciferase Reporter-Gene Assay on the SpectraMax M5, LMax II384 and Analyst GT Microplate Readers
Reporter-gene assays are used to study eukaryotic gene expression. Dual genetic reporters are commonly used in transient transfections of cultured cells to minimize experimental variability. Recently, Promega introduced a Dual-Glo Lucoferase assay system which replaced the outdated ‘flash’ assays used previously. Ensuring both genetic reporters have different substrate requirements enables them to be detected using a single reaction mixture.