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Application Note

3D Cell Culture: mimicking real tissue

Rectangle Image
Application Note

3D Cell Culture: mimicking real tissue

Experiments performed in a classical two-dimensional (2D) cell culture system have resulted in a large body of knowledge about basic life science.  However, the 2D cell culture systems used so far have several drawbacks: the morphology, proliferation, metabolism and expression profiles of cells grown in 2D systems are very different to cells in living tissues.


Alvetex Scaffold provides an in vivo proxy for performing a variety of cellular and molecular techniques and biochemical assays, resulting in more biologically relevant data. When combined with the Freedom EVO liquid handling platform, cell biologists will be able to achieve a higher throughput, as well as a higher level of standardization for both basic research and drug discovery. 


This study demonstrates that the Freedom EVO workstation can simply and routinely process cell cultures in Alvetex Scaffold in a 96-well plate format. 

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