Ensure Sample Integrity Over Time

azenta.com CONSUMABLES & INSTRUMENTS Maintaining Sample Integrity During Repeated Freeze/Thaw Cycles KobeBioRobotiX, Azenta Life Sciences TECH NOTE Azenta Life Sciences 2 CONSUMABLES & INSTRUMENTS TECH NOTE The Challenge Storage of biological materials or newly synthesized compounds in -80°C freezers is now commonplace. The need to retrieve aliquots from these compound libraries or bio stores means that individual tubes must first be thawed and, following the aliquoting procedure, re-frozen. For popular, interesting, or useful samples this can result in multiple freeze/thaw cycles during the storage life of the sample. This paper sets out to discover if samples stored in quality screw-cap sample tubes rated for -80°C storage suffer any degradation from either very long-term storage or repeated freeze/ thaw cycling. The Details To ascertain the facts about extended storage, representative sample storage tubes of four types were used and, in all cases, stored in -80°C freezer conditions. The testing had three main objectives: • To confirm any changes to the sample storage volume that may occur throughout its lifecycle in a freezer • To confirm no damage is likely to occur that could compromise the integrity of the tube during regular freeze/thaw cycles • To re-affirm that the dual-threaded cap design successfully prevents leakage and evaporation over long periods of time The tests were conducted on externally threaded tubes designed and manufactured for Azenta of 1.0 mL and 1.9 mL working volume, and 1.0 mL & 3.8 mL working volume treated with electron-beam (e-Beam) radiation. Apparatus & Materials Tubes selected for tests were Azenta Tri-coded tubes, External Thread, Pre-racked: • 48 x 1.9 mL Tri-coded tube, 48-format, External Thread, e-Beam treated, Capped • 48 x 3.8 mL Tri-coded tube, 48-format, External Thread, Uncapped • 96 x 1.0 mL Tri-coded tube, 96-format, External Thread, Uncapped • 96 x 1.0 mL Tri-coded tube, 96-format, External Thread, e-Beam treated, Capped • Either 1 x 48- or 1 x 96-format Azenta External Cap Carrier • 1 x -80°C Freezer • 1 x Torque-controlled capper • 1 x 12-channel pipettor • 1 x 500 mL bottle of Ringer’s Solution • 1 x Precision laboratory balance Figure 1. Test samples stored in -80°C freezer Azenta Life Sciences 3 CONSUMABLES & INSTRUMENTS TECH NOTE Method Using the 12-channel pipettor, the maximum working volume (1,085 µl) of Ringer’s solution was aspirated into each of 96 tubes in the SBS rack. The capper was adjusted to apply 0.08 Nm of torque to each cap as it was transferred from the cap carrier to its tube, capping it accordingly. Using the precision balance, each tube was weighed and the weight and rack position from A1 to H12 was recorded. A visual check of each tube was made for any possible damage, such as grazing or cracking of the polymer surface and the condition of the was noted in a log. The completed rack of 96 tubes was then transferred to the -80°C freezer. The tubes were then withdrawn from storage at -80°C, at defined predetermined intervals as follows: At each sampling interval designated above, the SBS rack of tubes was removed from the freezer. The rack was then left out on the bench until the tubes had returned to room temperature. After stability at room temperature was achieved, each tube was re-weighed, and data was recorded. At the same time, each tube was checked visually for any possible damage, grazing, or cracking, and anything noteworthy was recorded. After completion of all weighing and checks, the filled SBS rack of tubes was returned to the -80°C freezer. The process was then repeated for each of the tube types at each incremental time point over three years, except the e-Beam sterile versions, which were only sampled after one year. Results & Observations For the 1.0 mL Azenta Tri-coded tubes, the total average weight loss of all 96 tubes was 0.010% (equal to 0.018 mg) over the first two-week sampling period and only increased to a loss of 0.045% (equal to 0.088 mg) over the full threeyear test period. Table 1. Results for 1.0 mL in 96-position rack Time of Weighing Average % Change Before Storage 100% 2 Weeks -0.01% 1 Month -0.01% 3 Months -0.03% 6 Months -0.03% 1 Year -0.03% 2 Years -0.04% 3 Years -0.05% 2 Weeks 1 Month 3 Months 6 Months 1 Year 2 Years 3 Years Azenta Life Sciences 4 CONSUMABLES & INSTRUMENTS TECH NOTE There was no observed cracking, grazing, or other physical damage caused throughout the frozen storage period, nor by repeated freeze/thaw cycles over a threeyear period. This demonstrates the seal integrity of the 1.0 mL Tricoded tubes when correctly capped is adequate for long-term storage of aqueous-based substances, if the specified working volume is adhered to. Using the above method, the test was repeated with the larger 1.9 mL Tri-coded tubes in a 48-position SBS rack. The only experimental differences were that the volume aspirated – in this case 2,073 µl of Ringer’s solution to more closely match the correct working volume of this larger tube and the torque setting of 0.10 Nm for the larger tube and cap in use. The total average weight change of all 48 x 1.9 mL tubes was 0.006% (equal to 0.022 mg) over two weeks, and then only increased to 0.06% (equal to 0.264 mg) over three years. Table 2. Results for 1.9 mL in 48-position rack Figure 2. Variation between columns in the same 1.0 mL tubes in 96-position rack over time Time of Weighing Average % Change Before Storage 100% 2 Weeks -0.01% 1 Month 0.00% 3 Months -0.04% 6 Months -0.04% 1 Year -0.04% 2 Years -0.06% 3 Years -0.07% 100.200% 100.000% 99.800% 99.600% 99.400% 99.200% Before Storage 2 Weeks 1 Month 3 Months 6 Months 1 Year 2 Years 3 Years Column A (ave) Column B (ave) Column C (ave) Column D (ave) Column E (ave) Column F (ave) Column G (ave) Column H (ave) Azenta Life Sciences 5 CONSUMABLES & INSTRUMENTS TECH NOTE Figure 3. Variation between columns in the same 48-position rack of 1.9 mL over time Figure 4. Comparing 1.9 mL 48-position and 1.0 mL 96-position Tri-coded tubes Again, there was no observed cracking, grazing, or other physical damage caused throughout the freezing period, nor by repeated freeze/thaw cycles over a three-year period. 100.200% 100.000% 99.800% 99.600% 99.400% 99.200% Before Storage 2 Weeks 1 Month 3 Months 6 Months 1 Year 2 Years 3 Years Column A (ave) Column B (ave) Column C (ave) Column D (ave) Column E (ave) Column F (ave) 0.000% -0.010% -0.020% -0.030% -0.040% -0.050% -0.060% -0.070% 48 Well 96 Well % Weight Loss over 36 months 0 5 10 15 20 25 30 35 40 Azenta Life Sciences 6 CONSUMABLES & INSTRUMENTS TECH NOTE 100.100% 100.000% 99.900% 99.800% 99.700% 99.600% Before Storage 1 Year Column A (ave) Column B (ave) Column C (ave) Column D (ave) Column E (ave) Column F (ave) Column G (ave) Column H (ave) Column I (ave) Effect of Electron Beam Treatment In the second part of the experiment, attention was turned to polymer tubes which had been treated using a standard dose of 62 kGy of e-Beam radiation. Both 1.0 mL and 3.8 mL treated tubes were tested, the key difference being that these treated tubes are supplied already capped. The experimental method for both was thus modified to include an initial step of decapping the racked tubes with the handheld single tube capper/decapper specified above. There were only two data points for these tests: initial weight and residual weight after one year. The aspirated volumes in this case were: 1 mL tube = 1.085 mL 4 mL tube = 4.150 mL The observed weight change after one year was as follows: For the 96 x e-Beam treated 1.0 mL Tri-coded tubes, pre-capped, pre-racked, the total average weight change of all 96 tubes was 0.006% (equal to 0.012 mg) over one year. Figure 5. Variation between columns in the same sterile 48-position rack over one year For the 48 x e-Beam treated 3.8 mL Tri-coded tubes, pre-capped, pre-racked the total average weight change of all 48 tubes was 0.0002% (equal to 0.0016 mg) over one year. Figure 6. Comparison of weight change at individual tube positions on a 48-position rack 7.4 7.35 7.3 7.25 7.2 7.15 7.1 A1 A2 A3 A4 A5 A6 A7 A8 B1 B2 B3 B4 B5 B6 B7 B8 C1 C2 C3 C4 C5 C6 C7 C8 D1 D2 D3 D4 D5 D6 D7 D8 E1 E2 E3 E4 E5 E6 E7 E8 F1 F2 F3 F4 F5 F6 F7 F6 Before Storage After 1 Year Before Storage After 1 Year azenta.com © 2024 Azenta US, Inc. All rights reserved. All trademarks are property of Azenta US, Inc. unless otherwise specified. 42023-ATN 0724 CONSUMABLES & INSTRUMENTS TECH NOTE Again, there was no observed cracking, grazing, or other physical damage caused throughout the freezing period, nor by repeated freeze/thaw cycles over a one-year period. Conclusions After collection of three years of tube weight data for the 1.0 mL and 1.9 mL Azenta Tri-coded tubes, and annual data for the 3.8 mL and 1.0 mL Azenta e-Beam treated tubes, it suggests a similar trajectory and gives confidence to state that these products are suitable for long-term storage applications. The Importance of Cap Design & Material Selection Azenta Tri-coded tubes have both the cap and the tube body manufactured from the same polymer. This reduces potential leakage caused by the differential coefficients of expansion that can be seen when two different polymers are used. By standardizing on the same polymer for construction throughout, any expansion or contraction during freeze/thaw cycles is the same for both parts of the article and the possibility of any gap opening between seal and tube is minimized. It is important to state here that accurate capping to the specified torque values is necessary to avoid any irregular damage to the threads of both the tube and the screwcap, as well as guaranteeing a reproducible and leak-proof seal each time. The use of a double-start thread, which helps to prevent cross threading, and an ingenious compression seal design that also prevents over-tightening of the cap all contribute to the intrinsic integrity of the Azenta tube design and the very low losses seen over time as demonstrated above. For the 96 x e-Beam treated 1.0 mL Tri-coded tubes, pre-capped, pre-racked, the total average weight change of all 96 tubes was 0.006% (equal to 0.012 mg) over one year. Figure 7. Schematic of the compression sealing design of an externally threaded tube with tolerances in milimeters The polymer used for these tubes is a polypropylene material with very low levels of extractables and leachables, to not contaminate the stored samples, especially those stored in solvent such as DMSO. Although free from DNA, DNA/RNA-ase, endotoxins, and pyrogens, the e-Beam process can also help to ensure sterility in the supplied product. Other treatment types such as gamma irradiation and ethylene oxide gas exposure can also be used to gain this benefit. It is encouraging to also note that freeze/thaw cycles do not seem to cause any unnecessary damage to this type of quality sample storage tube if handled in a careful and appropriate manner, thus making them ideal for biobanking and compound management applications that require multiple access of samples over their lifetime.