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Transfection of Primary Human Hepatocytes

Primary Human Hepatocytes (PHH) are a standard tool for determining drug metabolism, transport, and toxicity in pre-clinical drug development. However, just as virtually all non-dividing primary cells, PHH are difficult to transfect while maintaining their differentiated and specialized functions, limiting the ability for researchers to study more detailed mechanisms of these critical processes.

Download this application note to understand:

  • How 4D-Nucleofector differs to conventional culture systems
  • The benefits of having a culture that sustains cells for longer periods of time