Ultrapure Water in Cell Cultivation
Water is a major component of all cell culture media and is, therefore, needed to prepare media, buffers and additives, as well as to serve many ancillary functions, such as heating, cooling, cleaning and rinsing. Contaminants can occur in many forms, such as bacteria, yeasts or molds. These contaminants are usually visible to the eye or by optical microscopy. However, contamination from chemicals or other biological agents may also affect growth, morphology or behavior of cultured cells, yet be undetectable to the unaided eye. Water used in cell cultures must, therefore, be free of microorganisms, endotoxins, inorganic ions and organic compounds.
Download this app note to discover a system that can:
- Produce enhanced growth in cultured cell lines
- Minimize the impurity content in water
- Be used to reconstitute media for use in cell line cultivation