Exosome Isolation

Here at AMSBIO, we have developed and optimized methods and tools for fast, scalable and reproducible Extracellular Vesicle (EV) purification, as well as addressing EV heterogeneity and high throughput solutions for biomarker discovery.

Although ultracentrifugation is still widely used, it tends to alter the vesicle shape and functionality.

It's time-consuming methodology and need for expensive equipment has meant that it is no longer considered the gold standard for EV isolation.

Our products however; offer fast, cost-effective solutions for all your EV isolation needs.

Tangential Flow Filters for EV concentration and purification

Tangential flow filtration (TFF) is a rapid and efficient method, usually used for separation and purification of biomolecules in industrial applications and can be successfully applied to isolate and separate extracellular vesicles. We provide three different typologies of filters suitable respectively for EV purification, EV concentration and EV size-based separation.

TFF-Simple: Filter for EV concentration and dialysis

TFF-Simple is a filter cartridge in hollow fibers made of polysulfone, which allows the particle concentration and the removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc..), prior to the EV purification.

Applications

  • Concentration of diluted fluid as cell media or urine prior to EV isolation
  • Easy removal of small molecules and ions from the EV preparation
  • EV dialysis and buffer exchange
  • High efficiency of EV isolation if coupled with SEC columns

Advantages

  • Washable
  • Reusable multiple times. Easy to use
  • Fast concentration of EV containing matrices

NameDatasheetPacksize Order
TFF-Easy - tangential flow filtration concentrator1 concentrator View
TFF-Easy - tangential flow filtration concentrator 55 concentrators View

TFF-Micro: Filter for large EV separation and purification

TFF-Micro is a filter able to separate large microvesicles (MVs) by size, avoiding the separation by centrifugation at 10000g, which often causes the loss of part of small EVs. TFF-Micro retains vesicles larger than 150-200 nm, whereas small EVs and circulating molecules pass in the permeate. Retained MVs can be recovered with a syringe in PBS buffer, without additional purification steps.

Applications

  • Separation, concentration and recovery of large EVs (>150 nm)
  • Large EV isolation from cell media, biofluids, plant extracts
  • Dialysis and desalting of large EVs
  • Suitable for large EV isolation from 5 ml of fluid

Advantages

  • Washable
  • Reusable multiple times
  • Sterile
  • Fast separation of EVs bigger than 150 nm

NameDatasheetPacksize Order
TFF-MV- tangential flow filter for large EV separation and recovery1 concentrator View

TFF-Exo: Fast EV purification

TFF-Exo is our next-generation filter which allows a rapid, reproducible and scalable purification of EVs, can be used on the lab bench for purifying small amount of samples (min 5 ml) or connected with a mechanical system for purifying larger volumes.
TFF-Exo is a filter cartridge made of polyethersulfone hollow fibers with pores of 50 nm size (cut off 300 kDa). The filter allows the purification of EVs and particles > 50 nm.

Applications

  • Separation, purification and concentration of EVs from conditioned media, biofluids, plant extracts
  • Buffer exchange and removal of unbound dye
  • Depletion of FBS from bovine EVs

Advantages

  • Washable
  • Reusable multiple times
  • Sterile

NameDatasheetPacksize Order
TFF-EV-Large tangential flow filter for EV purification1 concentrator View
TFF-EV-Small tangential flow filter for EV purification1 concentrator View

ExoSpin: Ultrafiltration concentrator

ExoSpin is a 2.5 ml non-stick ultrafiltration (UF) concentrator to enhance the recovery of extracellular vesicle (EVs) during the concentration or buffer exchange step. The ultrafiltration works in the opposite direction to the centrifugal force, providing higher particle recovery. The low protein binding membrane (polyether- sulphone) reduces the EV loss, compared to V-shape concentrators, and the reverse design of the EV-spinner ensures that the filter does not clog.

ExoSpin Products

NameDatasheetPacksize Order
EV-Spinner: Ultrafiltration concentrator, MWCO 100 kDa, 24 pcs.24 concentrators View

ExoUltra: Size exclusion chromatography

Size Exclusion Chromatography (SEC) is an efficient method for isolating and purifying Extracellular Vesicles (EVs) from different fluids, not affecting the original shape and functionality of the vesicles. Our range of ExoUltra SEC columns allow for EV purification from small (100 µl) to large volumes (up to 20 ml) of fluids. EV purification with our ExoUltra columns takes approximately 15 minutes allowing integration into any laboratory workflow.

 

Characteristics

  • New gel matrix for EV purity improvement
  • Purification up to 20 ml volume of fluid

Advantages

  • Easy and fast protocol (turnaround time approximately 15 minutes). Isolate EVs from small sample volumes
  • Reusable up to 5 times
  • Long term stability at 4°C

Applications

  • Extracellular vesicles isolation from cell media, biofluids and plant extracts
  • Purification of EVs from contaminants
  • Dye excess removal post EV labelling process

NameDatasheetPacksize Order
PURE-EVs Complete kit: Size exclusion chromatography column/ TFF-easy/ TFF-MV-1 ma x i column + 2 tff concentrator View
PURE-EVs PLUS: Size exclusion chromatography columns and TFF concentrator-5 columns + 1 TFF concentrator View
PURE-EVs: Size exclusion chromatography column10 columns View
PURE-EVs: Size exclusion chromatography column5 columns View
maxiPURE-EVs PLUS: Size exclusion chromatography column and TFF concentrator-1 ma x i column + 1 tff concentrator View
maxiPURE-EVs: Size exclusion chromatography column3 columns View
maxiPURE-EVs: Size exclusion chromatography column6 columns View
miniPURE-EVs: Size exclusion chromatography column10 columns View
miniPURE-EVs: Size exclusion chromatography column20 columns View

ExoUltra: Immunoaffinity isolation

Understanding and addressing extracellular vesicle heterogeneity has become essential for the EV field, especially because of the different roles that EVs have in pathological processes such as cancer, infection, and neurodegenerative diseases.

Our pre-coated ELISA immunoplates and our latex or magnetic immunobeads are perfect for the capture and enrichment of total or specific EV-subpopulations.

Applications

  • Multiple profiling of EV markers from both a single sample or screening a large number of samples
  • EV capture and quantification from human biofluids (plasma, serum, urine, saliva)
  • Immunocaptured EVs are suitable for downstream processes such as nucleic acid extraction

Advantages

  • Ready to use.
  • Long term storage (24 months).
  • No EV pre-purification required
  • Small sample amounts required (100 μl per well).
  • Flexibility and ease when designing a multiplexing assay.
  • Open platform for customized coating solutions.

NameDatasheetPacksize Order
Immunoplate for capture L1CAM positive exosomes1 plate View
Immunoplate for capture CD63 positive Exosomes1 plate View
Immunoplate for capture EphrinB positive exosomes1 plate View
Immunoplate for capture PLP1 positive Exosomes1 plate View
Immunoplate for capture TM9SF4 positive exosomes1 plate View
Immunoplate for capture of CD9 positive Exosomes1 plate View

ExoPure: Chemical isolation

Our ExoPure is a chemical precipitation based, fast and convenient method of Extracellular Vesicle isolation from bio-fluids, cell culture supernatants and plant extracts.

Samples are incubated on ice with our ExoPure solution so that EVs will precipitate following centrifugation. The obtained pellet can be re-suspended in PBS 1X or deionized water. Our user-friendly protocol is time-saving and does not require expensive laboratory equipment. These isolated vesicles are suitable for isolation of EV associated nucleic acids.

NameDatasheetPacksize Order
EXO-Prep for exosome isolation from Cell Media25 ml (25 reactions) View
EXO-Prep for exosome isolation from Plasma and serum5 ml View
EXO-Prep for exosome isolation from Urine30 ml (25 reactions) View