10 Tips for Quantifying Immunohistochemistry Staining
How To Guide Nov 29, 2018 | by By Alexandra Boussommier-Calleja, PhD
Human HeLa cancer cells in culture showing the nuclei in red and the tubulin component of the cytoskeleton in green. The blue staining is a mitotic checkpoint protein that remains in the cytoplasm until pro-metaphase when it is involved in regulating cell division. HeLa cells are derived from a human cervical cancer and can be propagated indefinitely in culture. Image credit: Matthew Daniels, Wellcome Images via The Cell Image Library
Immunohistochemistry (IHC) is a popular tool used to advance our understanding of fundamental biology. The role of different cell types in the onset of diseases or in drug responses can also be directly observed. As powerful as IHC is, the way in which you should extract information from your images is not always obvious.
Here we expose 10 tips to help you design a strategy for analyzing your IHC images. These general tips are intended to help you design your analysis, and can be applied to your image analysis software of choice.
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