How To Achieve High-Quality and Reproducible Flow Cytometry Data

How To Guide

Last Updated: July 29, 2022

Written by

Derek Davies

How To Achieve High-Quality and Reproducible Flow Cytometry Data content piece image

Flow cytometry is an extremely widespread technique in the biomedical field as it has a large range of applications and is applicable to many cell types. To run a successful and reproducible flow cytometry experiment there are many factors that need to be considered.

Download this guide to discover the stages involved in achieving high-quality and reproducible flow cytometry data.

The areas covered in this guide include:

Assay design
Sample preparation
Cytometer characterization and quality control
Running the sample and acquiring data
Data storage, analysis and presentation

Meet the Author

Derek Davies

Cytometry Consultant

Derek Davies has been involved in Cytometry for over 40 years, firstly as a researcher and then as the head of a large core facility, initially at the Cancer Research UK London Research Institute (1990-2015) and then at the Francis Crick Institute in London, UK (2015-2019). From 2019, Derek was the Science Technology Platform Training Lead at the Crick, looking after internal and external training across all core facilities. In that time, he has trained thousands of researchers to use analytical flow cytometers and cell sorters and advised on experimental design and data analysis. In 2023 he embarked on semi-retirement but also acts as an independent trainer and consultant in Flow Cytometry.