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Your Guide to Culturing Organoids

Organoid

Organoids are primary patient-derived micro tissues grown within a three-dimensional extracellular matrix. They offer a better representation of in vivo physiology than existing two-dimensional cell lines; however, organoid culturing requires additional considerations and tissue-specific formulations that may not be available commercially. 

This guide equips researchers with a standardized approach for routine handling of organoids, including protocols that can be applied to both normal and diseased tissue from various tissue types.

Download this guide to learn more about:

  • How to grow and expand organoids to study cells with complex organization and disease-state phenotypes
  • Cryopreservation of organoids and how to thaw them 
  • Critical parameters for success and how to troubleshoot common problems
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