Modern cell biology demands more than single-parameter measurements. The ability to continuously monitor living cells across multiple dimensions has become essential in drug discovery and translational research.
Traditional workflows that rely on managing multiple instruments, assay formats, and analysis pipelines are full of friction that slows discovery and undermines data confidence.
This infographic explores how seven integrated capabilities can be unified into a single experimental ecosystem, enabling more confident decision-making from early discovery through regulated manufacturing.
Download this infographic to explore:
- How combining impedance biosensing and live-cell imaging delivers complementary functional and visual readouts
- How AI-powered image analysis enables scalable, consistent insights
- How integrated compliance-supporting software controls maintain data integrity in regulated workflows
The Complete Cell Analysis Solution
The Agilent xCELLigence RTCA eSight platform combines technologies that provide researchers with
unprecedented biological insight. This infographic explores how its seven components integrate to
support research from discovery through manufacturing.
Note: eSight 1.4.0 provides technical controls that support 21 CFR Part 11 requirements.
Compliance is determined by customer-managed processes, including validation (IQ/OQ/
PQ), SOPs, training, IT governance, and data archiving.
Why Multiple Modalities Matter
Many traditional cellular workflows often rely on single-parameter approaches, offering a very specific lens
on behavior, but requiring multiple methods to build a complete understanding.
The need for efficient, multidimensional understanding has become especially critical with the adoption
of new approach methodologies (NAMs), which drive the use of more complex, human-relevant
cellular models in place of animal testing. Fully understanding these systems requires multiparametric
technologies capable of continuously monitoring living cells.
THE COMPLETE PICTURE
When these seven pieces work together, researchers unlock the power to investigate cellular behaviour in
the most comprehensive detail. The result is a workflow solution that supports confident decision-making
from early discovery through regulated manufacturing.
Seven Capabilities, One Integrated Solution
Impedance Biosensing
Impedance analysis measures changes in electrical resistance as cells interact with
microelectrodes. Processes that alter cell adhesion, morphology, or viability all impact
cell–electrode interaction, producing changes in current flow that can be continuously and
quantitatively monitored to track cellular responses to treatments.
Dual-Modality Integration
By pairing impedance measurements with live-cell imaging in the same well,
quantitative kinetic response profiles are directly connected to visual phenotypes,
supporting interpretation of cellular mechanisms, all within a single experiment.
AI-Powered Image Analysis
eSight AI helps researchers turn live-cell images into consistent, quantitative insights,
with less manual effort—supporting scalable experiments, improving consistency, and
increasing confidence in results.
Unified Experimental Ecosystem
Perform impedance biosensing and live-cell imaging in the same well, enabled by
advanced analysis—integrating acquisition, AI-driven insights, data comprehension, and
compliance-supporting workflows all from a single instrument.
Scalable Live-Cell Imaging
Capture morphological and phenotypic changes with spatial detail while imaging up
to five 96-well plates per run, supporting higher-throughput experiments and richer
interpretation of cellular responses.
Advanced 3D Model Support
Analyze complex 3D cell models with quantitative imaging that captures how spheroids
and organoids grow, change, and respond to treatments over time, providing cellular
insights in more physiologically and translationally relevant systems.
Compliance-Supporting Software Controls
Maintain secure, traceable cell analysis data with software controls designed to support
regulated laboratory workflows and data integrity requirements.
The RTCA eSight integrates seven core capabilities into a single environment, simplifying how complex
cellular model systems are understood.
eSight: Piece Together the Full Picture of Cellular Behavior
• Label-free evaluation of
cellular responses, enabling
simpler workflows and more
physiologically relevant results
• Continuous, real-time kinetic
measurement of cellular changes
• Sensitive, quantitative detection
of subtle cellular behaviors
• Two complementary
readouts, one well:
functional and visual
• Higher confidence in
data interpretation and
mechanistic insight
• One integrated workflow,
one instrument, and one
assay format for greater
efficiency
• Consistent, trustworthy results
across users, experimental
conditions, and time
• Scale imaging experiments
without increasing variability
or workflow drift
• Focus on biological insights,
not image analysis setup or AI
model training
• Functional and visual data
acquisition, analysis, AI-driven
insights, and compliance
support within one ecosystem
• Maintain stable culture
conditions by avoiding plate
transfers between instruments
• Better biology, better
experiments, simpler workflow
• Brightfield and multicolor
fluorescence imaging for
visual confirmation and
cellular context
• Time-resolved visualization
of morphological and
phenotypic changes
• Support for complex 2D
and 3D models
• Real-time, longitudinal
monitoring of spheroid
and organoid growth
• Quantitative analysis of
treatment responses in
3D models
• Visualization of structural
and phenotypic changes
as 3D cultures evolve
• Support for 21 CFR Part 11 and
Annex 11 aligned workflows
• Role-based access and
electronic approvals for
controlled data management
• Comprehensive audit trails
to track user activity, data
changes, and analysis steps
Impedance Imaging
Tracking
Real-time
monitoring
Screening
Ready To See the Full Picture? Explore the Latest Cell Analysis Tools Now
3D
3D
