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Adipocyte Development Spurred by Fracking Chemicals and Wastewater

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Adipocyte Development Spurred by Fracking Chemicals and Wastewater

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Exposure to fracking chemicals and wastewater promotes fat cell development, or adipogenesis, in living cells in a laboratory, according to a new Duke University-led study.

Researchers observed an increase in the size and number of fat cells after exposing living mouse cells in a dish to a mixture of 23 commonly used fracking chemicals. They also observed these effects after exposing the cells to samples of wastewater from fracked oil and gas wells and surface water believed to be contaminated with the wastewater.

“We saw significant fat cell proliferation and lipid accumulation, even when wastewater samples were diluted 1,000-fold from their raw state and when wastewater-affected surface water samples were diluted 25-fold,” said Chris Kassotis, a postdoctoral research associate at Duke’s Nicholas School of the Environment, who led the study.

“Rather than needing to concentrate the samples to detect effects, we diluted them and still detected the effects,” he said.

Previous lab studies by Kassotis and his colleagues have shown that rodents exposed during gestation to the mix of 23 fracking chemicals are more likely to experience metabolic, reproductive and developmental health impacts, including increased weight gain.

Kassotis said further research is needed to assess whether similar effects occur in humans or animals who drink or come into physical contact with affected surface waters outside the laboratory.

More than 1,000 different chemicals are used for hydraulic fracturing across the United States, many of which can act as endocrine-disrupting chemicals in both cell and animal models.

Kassotis and colleagues collected samples of fracking wastewater and wastewater-contaminated surface water near fracked oil and gas production sites in Garfield County, Colorado, and Fayette County, West Virginia, in 2014.

Laboratory cultures of mouse cells were then exposed to these waters at varying concentrations or dilutions over a two-week period. The researchers measured how fat cell development in the cultures was affected. They performed similar tests exposing cell models to a mix of 23 fracking chemicals.

Within each experiment, other cells were exposed to rosiglitazone, a pharmaceutical known to be highly effective at activating fat cell differentiation and causing weight gain in humans.

The tests also provided insights into the mechanisms that might be driving these effects.

“Activation of the hormone receptor PPAR-gamma, often called the master regulator of fat cell differentiation, occurred in some samples, while in other samples different mechanisms such as inhibition of the thyroid or androgen receptor, seemed to be in play,” Kassotis explained.

In this study, the 23-chemical mix induced about 60 percent of the fat accumulation as the potent pharmaceutical. The diluted wastewater samples and diluted surface water induced about 80 and 40 percent of the fat accumulation induced by rosiglitazone, respectively.

In all three cases, the number of pre-adipocytes, or precursor fat cells, that developed was much greater in cell models exposed to the chemicals or water samples than in those exposed to rosiglitazone.

This article has been republished from materials provided by Duke University. Note: material may have been edited for length and content. For further information, please contact the cited source.


Reference

Kassotis, C. D., Nagel, S. C., & Stapleton, H. M. (2018). Unconventional oil and gas chemicals and wastewater-impacted water samples promote adipogenesis via PPARγ-dependent and independent mechanisms in 3T3-L1 cells. Science of The Total Environment. doi:10.1016/j.scitotenv.2018.05.030

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