We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Cell Line Successfully Transitioned to Animal-Free Media
News

Cell Line Successfully Transitioned to Animal-Free Media

Cell Line Successfully Transitioned to Animal-Free Media
News

Cell Line Successfully Transitioned to Animal-Free Media

Credit: Pixabay
Read time:
 

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "Cell Line Successfully Transitioned to Animal-Free Media"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

A new paper by PETA Science Consortium International e.V. and the Luxembourg Institute of Science and Technology shows how to improve the relevance and reproducibility of research by replacing media containing serum extracted from the blood of unborn calves with animal-free media.

An estimated 1.8 million unborn calves are killed worldwide to produce fetal bovine serum (FBS)—an undefined mixture of molecules, including hormones, proteins, and growth factors obtained from the blood of fetal calves after their mothers are slaughtered for food. FBS, which is used in laboratories to help grow cells in vitro, is a source of variability, contributing to the reproducibility problem in research. Scientific organizations, such as the Organisation for Economic Co-operation and Development and the European Union Reference Laboratory for Alternatives to Animal Testing, have encouraged the transition from using FBS to using animal-free, chemically defined media.

The paper, published in Toxicology in Vitro, describes the transition of A549 cells, a human lung cell line commonly used in research, to cell culture media without FBS or any other animal-derived components. The success of this project sets a precedent for making the same transition for other cell types and has far-reaching implications for the field of in vitro research.

“Our study demonstrates the feasibility of using animal-free media, and it provides a template that can be replicated to transition other cell types to serum-free media,” says Science Consortium President Dr. Amy Clippinger. “The use of animal-free media has numerous advantages, and we encourage researchers to invest in this opportunity to increase the quality and reproducibility of studies.” 


Reference: Chary A, Groff K, Stucki AO, et al. Maximizing the relevance and reproducibility of A549 cell culture using FBS-free media. Toxicology in Vitro. 2022;83:105423. doi: 10.1016/j.tiv.2022.105423


This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.


Advertisement