Cryopreserved Reticulocytes Derived from Stem Cells can be Invaded by Cryopreserved Plasmodium vivax Isolates
News Nov 07, 2012
The development of a system for the continuous culture of Plasmodium vivax in vitro would benefit from the use of reticulocytes derived from differentiated hematopoietic stem cells (HCS). At present, the need to use both fresh reticulocytes and fresh P. vivax isolates represents a major obstacle towards this goal, particularly for laboratories located in non-endemic countries. Here, we describe a new method for the cryopreservation of HSC-derived reticulocytes to be used for both P. falciparum and P. vivax invasion tests. Cryopreserved P. falciparum and P. vivax isolates could invade both fresh and cryopreserved HSC-derived reticulocytes with similar efficiency. This new technique allows the storage of HSC-derived reticulocytes which can be used for later invasion tests and represents an important step towards the establishment of a continuous P. vivax culture.
The article is published online in the journal PLoS ONE and is free to access.
3rd International Conference On Agricultural Engineering and Food Security
Nov 12 - Nov 13, 2018
25th International Conference on Advanced Clinical Research and Clinical Trials
Sep 16 - Sep 17, 2019
8th InterAmerican Oncology Conference 'Current Status and Future of Anti-Cancer Therapies'
Oct 17 - Oct 18, 2019