We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Raman Spectroscopy and CARS Microscopy of Stem Cells and Their Derivatives
News

Raman Spectroscopy and CARS Microscopy of Stem Cells and Their Derivatives

Raman Spectroscopy and CARS Microscopy of Stem Cells and Their Derivatives
News

Raman Spectroscopy and CARS Microscopy of Stem Cells and Their Derivatives

Read time:
 

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "Raman Spectroscopy and CARS Microscopy of Stem Cells and Their Derivatives"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Abstract
The characterisation of stem cells is of vital importance to regenerative medicine. Failure to separate out all stem cells from differentiated cells before therapies can result in teratomas - tumours of multiple cell types. Typically, characterisation is performed in a destructive manner with fluorescent assays. A truly non-invasive method of characterisation would be a major breakthrough in stem cell-based therapies. Raman spectroscopy has revealed that DNA and RNA levels drop when a stem cell differentiates into other cell types, which we link to a change in the relative sizes of the nucleus and cytoplasm. We also used Raman spectroscopy to investigate the biochemistry within an early embryo, or blastocyst, which differs greatly from colonies of embryonic stem cells. Certain cell types that differentiate from stem cells can be identified by directly imaging the biochemistry with CARS microscopy; examples presented are hydroxyapatite - a precursor to bone, and lipids in adipocytes.

The article is published in the Journal of Raman Spectroscopy and is free to access.

Advertisement