We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.


Recipe for Making Blood Vessel Cells From Patient Stem Cells Published

Red blood cells in a blood vessel.
Credit: Narupon Promvichai/ Pixabay

Want a FREE PDF version of this news story?

Complete the form below and we will email you a PDF version of "Recipe for Making Blood Vessel Cells From Patient Stem Cells Published"

Listen with
Register for free to listen to this article
Thank you. Listen to this article using the player above.
Read time:

Researchers at NIH’s National Eye Institute have published a detailed protocol for making three cell types that are key components to form blood vessels and capillaries. The technique enables researchers to make tissues for study and potentially for future treatments.

Want more breaking news?

Subscribe to Technology Networks’ daily newsletter, delivering breaking science news straight to your inbox every day.

Subscribe for FREE

“This protocol generates vascular endothelial cells, pericytes, and fibroblasts from human induced pluripotent stem cells, called iPSCs,” said Tea Soon Park, Ph.D., NEI Ocular and Stem Cell Translational Research Section, and lead author of the protocol.

IPSCs are made in a lab, starting with skin or blood cells. They can then be modified to create nearly any other cell type of the body. 

“Since all three cell types in this protocol are major parts of vascular structure, it will be a useful tool to study a variety of diseases including eye disease,” said Park.

The five-step protocol, from iPSCs to fibroblasts, takes about four weeks. It includes seeding of iPSCs, seven days of monolayer differentiation, and cell separation based on CD31 expression. CD31 is a cell-surface protein unique to endothelial cells. At the end of step 3, endothelial and pericyte populations are separated. Fibroblasts can then be further differentiated from pericytes.

Park and colleagues are now working on a method to combine the three patient-derived cell types using bioprinting, which distributes and orients cells in a 3D space resembling the tissue microenvironment. 

Reference: Park TS, Hirday R, Ali A, et al. Protocol to generate endothelial cells, pericytes, and fibroblasts in one differentiation round from human-induced pluripotent stem cells. STAR Protoc. 2023;4(2):102292. doi: 10.1016/j.xpro.2023.102292

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.