Spees Research Identifies Promising Tool for Cardiac Stem Cell Grafting
News Dec 17, 2013
Stem cells, which can come from embryos, fetal tissue and adult tissues, have the potential to develop into a variety of cell types in the body, such as muscle cells, brain cells and red blood cells. These cells also possess the ability to repair human tissues. The field of regenerative medicine – which explores the viability of using embryonic, fetal and adult stem cells to repair and regenerate tissues and organs – has struggled to successfully graft cells from culture back into injured tissue.
“Many grafts simply didn’t take; the cells wouldn’t stick or would die,” explains Spees. So he and his research team set out to develop ways to enhance graft success.
They focused on a type of bone marrow-derived progenitor cell that forms stromal cells. Stromal cells form connective tissue and also support the creation of blood cells. The researchers were aware of that these cells secrete a diverse array of molecules – called ligands – that protect injured tissue, promote tissue repair and support stem and progenitor cells in culture. Different ligands interact with specific receptors on the surface of a stem or progenitor cell, transmitting signals that can instruct the cell to adhere, to divide, or to differentiate into a mature functional cell.
To confirm whether or not these types of ligands would protect a cardiac progenitor cell and help it graft, the group isolated a conditioned medium from human bone marrow-derived progenitor cells. They found that the medium contained Connective Tissue Growth Factor (CTGF) and the hormone insulin.
“Both CTGF and insulin are protective,” says Spees. “Together, they have a synergistic effect.”
In the study, Spees and colleagues compared the impact of sending a cardiac stem cell “naked” into a rodent heart with infarction (heart attack) to a cell that instead wore a “backpack” of protective ligands, created by incubating about 125,000 cardiac cells in a “cocktail” of CTGF and insulin on ice for 30 minutes. The team grafted the cells sub-epicardially – between the outer layer and the muscle tissue of the heart – and found that their priming cocktail resulted in improved graft success.
“We broke the record for engraftment!” exclaims Spees, who adds that the team’s priming tool –named “Cell-Kro” – dramatically increases cell adhesion, proliferation, survival, and migration.
While Spees and his colleagues continue to study grafting with cardiac stem cells from rats, the future could include human cardiac stem cells and human trials.
“There are about 650,000 bypass surgeries annually,” says Spees. “These patients could have cells harvested at their first surgery and banked for future application. If they return for another procedure, they could then receive a graft of their own cardiac progenitor cells, primed in Cell-Kro, and potentially re-build part of their injured heart.”
Switch Discovered to Convert Blood Vessels to Blood Stem Cells During Embryonic DevelopmentNews
Findings could aid research into creating new blood cells for transplants and understanding cancer metastasis.READ MORE
Chemicals in Lavender and Tea Tree Oil Could be Hormone DisruptorsNews
A new study lends further evidence to a suspected link between abnormal breast growth in young boys--called prepubertal gynecomastia--and regular exposure to lavender or tea tree oil, by finding that key chemicals in these common plant-derived oils act as endocrine-disrupting chemicals.READ MORE
Unraveling How Mesenchymal Stem Cells From Gum Tissue Accelerate Wound HealingNews
To assist with wound healing, mesenchymal stem cells from the gum tissue secrete extracellular vesicles that contain the anti-inflammatory signaling molecule IL-1RA.READ MORE
Comments | 0 ADD COMMENT
World Congress on Advanced Pharmacy and Clinical Research
Jul 16 - Jul 17, 2018
6th Annual Congress on Biology and Medicine of Molecules
Sep 17 - Sep 18, 2018
International Conference on Molecular Biology and Stem Cells
Aug 13 - Aug 15, 2018
World Congress on Plant Science and Molecular Biology
Sep 12 - Sep 13, 2018