Tecan’s NanoQuant™ Plate Gets under the Skin of Stem Cells
News Apr 10, 2009
Researchers in the Department of Biosciences and Nutrition at the Karolinska Institute in Stockholm, Sweden, have been using Tecan’s Infinite® 200 microplate reader and NanoQuant Plate™ as one of the methods for the characterization of a newly isolated population of skin stem cells in mice (Lgr5+ cells).
Dr Maria Kasper, from the Karolinska Institute’s environmental toxicology group, explained how the system is helping the research: “The scarcity of Lgr5+ cells makes the extracted genetic material extremely valuable, so we need a method to achieve exact quantification of this RNA with minimal sample loss. Using our Infinite reader with the NanoQuant Plate ensures that only minimal material is required for reliable evaluation of our valuable RNA samples, and allows us to investigate more than 30 selected genes by qPCR from just 150 ng of isolated RNA.”
Maria’s colleague, Dr Viljar Jaks, continued: “Because the NanoQuant Plate is integrated into the Infinite microplate reader, it is very easy to use and the i-control™ software is intuitive. Having it in the laboratory has been very useful for our investigations.”
In a new study in cells, University of Illinois researchers have adapted CRISPR gene-editing technology to cause the cell’s internal machinery to skip over a small portion of a gene when transcribing it into a template for protein building. This gives researchers a way not only to eliminate a mutated gene sequence, but to influence how the gene is expressed and regulated.
Researchers published today a detailed description of the complete genome of bread wheat, the world's most widely-cultivated crop. This work will pave the way for the production of wheat varieties better adapted to climate challenges, with higher yields, enhanced nutritional quality and improved sustainability.