Identification of New Hit Compounds Using a High-Throughput Phenotypic Screen with SMA Patient iPSC-Derived Motor Neurons
Spinal muscular atrophy (SMA) is an inheritable cause of infant mortality that is characterized by the loss of lower motor neurons and skeletal muscle atrophy. The degeneration of motor neurons is caused by insufficient levels of survival motor neuron (SMN) protein, which is encoded by two nearly identical genes SMN1 and SMN2. Most cases of SMA harbour homozygous deletions of the SMN1 gene and retain at least one copy of SMN2.
Optimized Conditions for MEA Assay with Human iPSC-Derived Spinal Motor Neurons, Glutamatergic Neurons and Mixed Cortical Neurons
In order to generate a robust protocol for MEA recording on hiPSC- derived neurons, we evaluated several conditions, which could affect culture performance (1.neuron seeding density; 2.seeding medium; 3.astrocyt eco-culture). These conditions were evaluated with BrainXell’s hiPSC-derived spinal motor neurons, cortical glutamatergic neurons and mixed cortical neurons.
ALS Drug Discovery via High-Throughput Phenotypic Screening Using iPSC-Derived Human Motor Neurons
At BrainXell, we established new technologies to rapidly differentiate ALS patient induced pluripotent stem cells (iPSCs) into large quantities of neurons.
TMT Quantification for Shotgun Proteomics: TIMS-QTOF Powered by PASEF
Download this free poster to learn how a unique dual trapped ion mobility spectrometer (TIMS) coupled to a QTOF (timsTOF Pro) can be used for the analysis of isobaric labeling experiments.
PASEF Enhances the Speed, Sensitivity and Selectivity of Label-free Quantification
Download this poster to learn how an adapted version of PEAKS™ enables identifications and label-free quantification from PASEF runs and enhances the speed, sensitivity and selectivity of label-free quantification.
Choosing the Right Parameters for Your UV-Vis Measurement
The Agilent Optimum Parameters Poster is a handy wall chart that provides information about choosing solvents, cuvette types, and managing your instrument parameters.
Applying QbD in Process and Impurity Control Strategy Development
Manage impurity data in pharmaceutical development more effectively in a single informatics solution. Assemble all relevant analytical data with chemical information for real-time access to project information by the entire team.
Detection of 17 Targets in a Single PCR Tube by a Novel MeltPlex® Probe System Combining Melting Curves and Taqman Probes
Despite the developments in conventional PCR, the complexity of multiplex Real Time PCR is still limited due to the lack of sufficient detection channels. To achieve high-end multiplexing capacity on standard Real Time PCR machines, Anapa Biotech has developed the MeltPlex® technology (see box on right).
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cells
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer cluster
P450 Induction in Cryopreserved Hepatocytes from PXR and CAR Nuclear Receptor Knock-out Rats
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.