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Latest Posters
Combining a Real-Time In Vitro Cell Viability Assay and RNA Extraction from the Same 3D Spheroids
Poster

Combining a Real-Time In Vitro Cell Viability Assay and RNA Extraction from the Same 3D Spheroids

We report here the results of using a novel small molecule probe that viable cells convert into a substrate for a shrimp-derived luciferase to generate a signal proportional to the number of viable cells.
Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)
Poster

Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)

The method allows for automated determination of total fat content and quantitative analysis of saturated and unsaturated cis- and trans- fatty acids of different chocolate products.
CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluation
Poster

CiPA Phase 2 Study: validation of an automated microelectrode array (MEA) assay of hiPSC-derived cardiomyocyte electrophysiology for cardiac safety evaluation

These results support the use of hSC-CM and MEA technology for preclinical assessment of proarrhythmic risk within the proposed CiPA paradigm, and, more generally, demonstrate that automation of the CM-MEA assay can achieve high reliability and throughput for cardiac risk assessment in vitro.
Monitoring Cell Health in Real-time / Time-lapse Cell-based Assays
Poster

Monitoring Cell Health in Real-time / Time-lapse Cell-based Assays

The purpose here is to validate a novel far-red DNA binding viability probe, DRAQ7™, in real-time cell based assays for 2-D and 3-D models.
Cell X and Colonyze Platform for Cell Sourcing and Process Controls
Poster

Cell X and Colonyze Platform for Cell Sourcing and Process Controls

Cleveland Clinic and Parker Hannifin have collaborated to create the Cell X and Colonyze platform as resource tools for cell source management and quality control.
Optogenetics on FDSS7000EX: Light-stimulated membrane depolarization in cultured cells expressing channelrhodopsin using a kinetic plate reader
Poster

Optogenetics on FDSS7000EX: Light-stimulated membrane depolarization in cultured cells expressing channelrhodopsin using a kinetic plate reader

The aim of this study was to establish clonal cell lines stably expressing a channelrhodopsin variant and measure membrane potential changes with a fluorescent dye induced by repetitive light-stimulations in a 96-well plate on a kinetic plate reader FDSS 7000EX.
Hexachlorophene reduces Tau aggregation and potential therapeutic agent for treatment of Alzheimer’s disease
Poster

Hexachlorophene reduces Tau aggregation and potential therapeutic agent for treatment of Alzheimer’s disease

An increase in phosphorylation of tau (hyper phosphorylation) leads to aggregation of the protein resulting in neurofibrillary tangles, a pathological hallmark associated with Alzheimer’s disease (AD). The identification of pharmacological agents that can help decrease levels of phosphorylated tau would be advantageous. Recent work in our laboratory has found that the molecule hexachlorophene can regulate levels of tau in cellular models.
Measuring dynamic and real-time cAMP levels using cADDis2, a live-cell indicator for Gs and Gi signaling
Poster

Measuring dynamic and real-time cAMP levels using cADDis2, a live-cell indicator for Gs and Gi signaling

Here, we introduce a new fluorescent cAMP indicator, cADDis2, capable of detecting dynamic changes in cAMP concentration in living cells.
Microfluidic Extrusion of Cell-laden Hydrogel Fibers for 3D-Bioprinting
Poster

Microfluidic Extrusion of Cell-laden Hydrogel Fibers for 3D-Bioprinting

An overview on how the incorporation of microfluidic platforms in 3D-bioprinters can open new routes in the creation of heterogeneous, tissue-specific bio-constructs and in the high-throughput screening of bioinks compositions for regenerative medicine and in vitro tissue modeling.
Comparison of Key Signaling Pathways in Lung Cancer Cells Grown in 2D and 3D Formats
Poster

Comparison of Key Signaling Pathways in Lung Cancer Cells Grown in 2D and 3D Formats

Evaluation of Wnt/ß-catenin and BMP pathway proteins and gene expression in NSCLC cell lines A549 and H460 in 2D and 3D cultures.

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