A novel Hydrogel Mountant - CyGEL™ - Enables Temporospatial HCS Imaging Assays of Live non-Adherent Cells
Poster Apr 25, 2007
Edward R, Errington RJ, Patterson LH, Ogrodzinski S and Smith PJ
Imaging-based assays have primarily relied upon adherent cell types for temporospatial measurements. This limits choice of cell type and extension of detection technologies to relevant biological models that may comprise nonadherent (e.g. suspension culture) or indeed ‘detaching’ (e.g. loss of viability/adherence) cell states such as drug candidate screening in immunological and lymphoproliferative disorder models, where the therapeutic target is often presented by a non-adherent type cell or where a biological response requires changes in adherence to a substrate. New technologies are thus needed to deal with live cell-based HCS assays on non-adherent cells or to exploit suspension cells in fluidic systems.
Here we describe CyGEL™ - a thermo-reversible hydrogelbased 4-D immobilization technology for live cell location without imposing an anchoring to a substrate.
Exploiting Polypharmacology in Precision Oncology: Identification of Differential Kinase Off-targets Among Clinical PARP InhibitorsPoster
Can we use computational methods to identify previously unknown off-targets of PARP inhibitors that can explain their observed differences?READ MORE
Bioluminescent Assay for GTPases Allows Measurement of GTPase, GAP and GEF ActivitiesPoster
We have developed a homogenous bioluminescent assay (GTPase-Glo) system to analyze these proteins in a simple, convenient “add-mix-read” format.READ MORE
Fc Effector Bioassays for Rapid and Quantitative Measurement of ADCC and ADCP Mechanisms of ActionPoster
Drug developers are rapidly adopting Fc effector function reporter-based bioassays to measure antibody Fc functions such as ADCC and ADCP activity during the development of therapeutic antibodies. Here we show application of a suite of FcgR Reporter Bioassays to elucidate and characterize antibody MOA.READ MORE