Adaptive control of glycerol & methanol feeding in recombinant Pichia pastoris cultures: Impact on antibody titre
Poster Aug 18, 2008
Pichia pastorisprocesses for heterolougous protein expression are typically run in three phases: a batch phase, an exponential feeding fed-batch phase and a long oxygen transfer limitation (OTL) phase. The final protein titre is often limited by the oxygen availability in the OTL phase. Here a direct adaptive controller is designed for the OTL phase. The controller was validated in pilot P. pastoris cultivations expressing a single chain antibody fragment (ScFv). This work shows that the proposed controller can regulate the dissolved oxygen tension (DOT) at very low levels (5 %) with high stability by manipulating the glycerol feeding rate, thereby enhancing the oxygen transfer at high cell density, which finally leads to a higher antibody titre.
LC-SWATH/MS Metabolomics Platform with Hyphenation of Extraction for the Analysis of Polar and Non-polar Metabolites in Biological SamplesPoster
Automated robotic sample preparation with hyphenated dual LC-SWATH/MS analysis for lipids and polar metabolites.
Quantitative Live-Cell Analysis Using Automated Long-Term ImagingPoster
Here we describe a continuous live-cell assay for determining cell proliferation profiles using the BioSpa Automated Live Cell Imaging System, consisting of BioSpa 8 and Cytation 5.READ MORE