DNA Methylation Analysis – Reliable Cell Characterization in Regenerative Medicine
Poster Apr 16, 2007
We demonstrate that DNA methylation patterns can serve as characteristic markers to distinguish different cell types. We have identified panels of methylation markers that are specific to mesenchymal stem cells or various differentiated cell types in the mesenchymal lineage. This method of cell type identification has a number of advantages over conventional markers in that it is robust, is both qualitative and quantitative.
Genomic Analysis of L5178Y tk+/- Cells and their Induced tk-/- Mutant ColoniesPoster
The goal of this pilot study was to exploit NGS technology with a view to shedding new light on the genome of cells used in the MLA, as well as the changes induced as a result of the exposure to mutagens.READ MORE
Applications of chemically modified synthetic guide RNA for CRISPR-Cas9 genome editingPoster
Our results indicate that MS modifications are required for experiments with co-electroporation of Cas9 mRNA and synthetic gRNA, yet have no impact on editing efficiency when delivered with lipid-based transfection reagents.READ MORE
Highly Accurate HCV Genotyping by Targeted Next Generation SequencingPoster
The recent fast advancement of next generation sequencing (NGS) technologies allowing for unprecedented speed and accuracy in analyzing viral genomes are opening new ways to further improve diagnostic genotyping of HCV.