High Content Analysis of Neural Stem Cell Expansion and Differentiation
Poster Dec 19, 2011
Oksana Sirenko, Allan C. Powe, Steven L. Stice, Karen Cook, Nick Callamaras, Jayne Hesley, Xin Jiang and Evan F. Cromwell
High content imaging methods that allow automatic evaluation of proliferation and
differentiation of hESC derived neuronal progenitors have been described.
The following effects of positive and negative factors on neuronal development have been demonstrated.
- EPO promotes differentiation of human neural progenitors toward neurons.
- No inducer SNAP, Antimycin A and staurosporine inhibit neuronal development.
These methods can be used to automate assays for:
- Testing biologics or chemical compounds on neuronal development
- Screening and validation of drug candidates
- Evaluating potential neurotoxic effects of different agents
Stem cell derived neurons and high content imaging is known to provide a powerful platform for neurobiology research and drug screening.
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