Improved Expansion of Neural Stem Cells with Gibco™ Heat Stable Recombinant Human Basic Fibroblast Growth Factor
Poster May 19, 2021
Brittany Balhouse, Diana Navarro, Richard Josephson, and Matthew Dallas
Basic fibroblast growth factor (bFGF) is used in neural stem cell (NSC) media to maintain multipotency. Native bFGF rapidly loses biological activity when exposed to culture conditions (37°C), Heat Stable (HS) bFGF maintains > 90% homology to the native protein and ≥ 80% biological activity, even after 72 hours of exposure to 37°C. In human embryonic stem cell-derived NSCs, HS bFGF can maintain multipotency and standard doubling times with reduced bFGF concentrations. In primary rat NSCs, using HS bFGF allows for a more user-friendly workflow without the loss of multipotency or slower proliferation. After expansion, HS bFGF can be removed just as easily as native bFGF to allow for downstream differentiation into neurons and glial cells.
For circulating cell free DNA (ccfDNA) to be used in cancer research successfully, workflow standardization is essential. Access this poster to discover tips on optimal workflow control, how to yield smaller ccfDNA fragments and the differences in quantification and qualification of ccfDNA.READ MORE