Measuring dynamic and real-time cAMP levels using cADDis2, a live-cell indicator for Gs and Gi signaling
Poster Jan 25, 2017
Paul Tewson, Scott Martinka, Catherine Berlot, Thom Hughes, Anne Marie Quinn & Shane Tillo
Here, we introduce a new fluorescent cAMP indicator, cADDis2, capable of detecting dynamic changes in cAMP concentration in living cells. We have applied cADDis2 to use in a novel Gi assay that, for the first time, directly reports Gi mediated reductions in cAMP concentration, across a variety of receptors, on standard automated fluorescence plate readers.
Bioluminescent Assay for GTPases Allows Measurement of GTPase, GAP and GEF ActivitiesPoster
We have developed a homogenous bioluminescent assay (GTPase-Glo) system to analyze these proteins in a simple, convenient “add-mix-read” format.READ MORE
Validation of an Image-Based 3D Natural Killer Cell Mediated Cytotoxicity AssayPoster
Here we describe a novel 3D NK cell mediated cytotoxicity (CMC) assay.READ MORE
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NanoBRET PPI System is composed of two components.
NanoBRET offers improved S:B over other BRET assays.
NanoBRET provides sensitive live-cell method to study protein interactions.
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