Measuring dynamic and real-time cAMP levels using cADDis2, a live-cell indicator for Gs and Gi signaling
Poster Jan 25, 2017
Paul Tewson, Scott Martinka, Catherine Berlot, Thom Hughes, Anne Marie Quinn & Shane Tillo
Here, we introduce a new fluorescent cAMP indicator, cADDis2, capable of detecting dynamic changes in cAMP concentration in living cells. We have applied cADDis2 to use in a novel Gi assay that, for the first time, directly reports Gi mediated reductions in cAMP concentration, across a variety of receptors, on standard automated fluorescence plate readers.
Primary Human Hepatocyte (PHH) culture provides the closest in vitro model to human liver that can produce a metabolic profile of a given drug very similar to that found in vivo. Recently we have developed an easy-to-assemble user-friendly in vitro Primary Human Hepatocyte (PHH) 3D-spheroid modelREAD MORE