Measuring dynamic and real-time cAMP levels using cADDis2, a live-cell indicator for Gs and Gi signaling

Here, we introduce a new fluorescent cAMP indicator, cADDis2, capable of detecting dynamic changes in cAMP concentration in living cells. We have applied cADDis2 to use in a novel Gi assay that, for the first time, directly reports Gi mediated reductions in cAMP concentration, across a variety of receptors, on standard automated fluorescence plate readers.