Monitoring Cell Health in Real-time / Time-lapse Cell-based Assays
Poster Feb 21, 2017
The purpose here is to validate a novel far-red DNA binding viability probe, DRAQ7™, in real-time cell based assays for 2-D and 3-D models. This requires DRAQ7™ to neither synergise nor interfere with toxicants / anti-cancer compounds. It must be capable of addition at any stage of an assay to permit real-time monitoring of membrane integrity loss, retaining its functionality throughout.
As background, DRAQ7™’s DNA-specificity allows monitoring cell-by-cell while spectral properties permit incorporation in multi-colour experiments e.g. with vital probes, mitochondrial health probes or Annexin V. DRAQ7™ has previously been shown to be truly cell impermeant yet retains DNA binding and far-red fluorescence of the DRAQ chromophore which makes it compatible with HCS imagers, fluorescence microscopes and cytometers. The excitation/emission properties are particularly suited to penetrative imaging of multi-cellular structures and thick ex-plant tissue sections, whilst limiting risks of short wavelength DNA damage.
Assessment of Oral LISPRO Treatment in Ameliorating Amyloid and Tau Pathology in Transgenic Alzheimer’s Mice ModelPoster
Ionic co-crystals of lithium salicylate with organic proline (LISPRO) showed better safety and pharmacokinetic profile of lithium in plasma and brain of wild-type and transgenic Alzheimer mice model compared to lithium salts.READ MORE
Combining a Real-Time In Vitro Cell Viability Assay and RNA Extraction from the Same 3D SpheroidsPoster
We report here the results of using a novel small molecule probe that viable cells convert into a substrate for a shrimp-derived luciferase to generate a signal proportional to the number of viable cells.READ MORE
Determination of the Fat Content Profile of different Chocolate Products using an Automated Workflow for the Generation of Fatty Acid Methyl Esters (FAME)Poster
The method allows for automated determination of total fat content and quantitative analysis of saturated and unsaturated cis- and trans- fatty acids of different chocolate products.READ MORE
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Outsourcing in Clinical Trials Southern California
Sep 27 - Sep 28, 2017