Monitoring Cell Health in Real-time / Time-lapse Cell-based Assays
Poster Feb 21, 2017
The purpose here is to validate a novel far-red DNA binding viability probe, DRAQ7™, in real-time cell based assays for 2-D and 3-D models. This requires DRAQ7™ to neither synergise nor interfere with toxicants / anti-cancer compounds. It must be capable of addition at any stage of an assay to permit real-time monitoring of membrane integrity loss, retaining its functionality throughout.
As background, DRAQ7™’s DNA-specificity allows monitoring cell-by-cell while spectral properties permit incorporation in multi-colour experiments e.g. with vital probes, mitochondrial health probes or Annexin V. DRAQ7™ has previously been shown to be truly cell impermeant yet retains DNA binding and far-red fluorescence of the DRAQ chromophore which makes it compatible with HCS imagers, fluorescence microscopes and cytometers. The excitation/emission properties are particularly suited to penetrative imaging of multi-cellular structures and thick ex-plant tissue sections, whilst limiting risks of short wavelength DNA damage.
The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that regulate the expression of phase I (cytochrome P450s), phase II metabolizing enzymes and transporter genes in response to xenobiotics, including prescription drugs.READ MORE
We show that network analysis of co-localized ions from mass spectrometry imaging data provides a detailed chemo-spatial insight into the metabolic heterogeneity of tumors. Furthermore, module preservation analysis between colorectal cancer patients with and without metastatic recurrence suggests hypotheses on the nature of the different local metabolic pathways.READ MORE
This abstract discusses three cases of pediatric heart transplant patients who suffered from parvovirus (B19) infection. Of these patients, two ( B & C) responded well to standard intravenous Ig therapy. Patient A however, did not respond to standard treatment and was begun on subcutaneous Ig, which effectively diminished his viral load. Thus, subcutaneous Ig infusions might serve as a second line treatment for transplant patients with parvovirus who do not respond well to the standard approach.READ MORE