Monitoring Cell Health in Real-time / Time-lapse Cell-based Assays
Poster Feb 21, 2017
The purpose here is to validate a novel far-red DNA binding viability probe, DRAQ7™, in real-time cell based assays for 2-D and 3-D models. This requires DRAQ7™ to neither synergise nor interfere with toxicants / anti-cancer compounds. It must be capable of addition at any stage of an assay to permit real-time monitoring of membrane integrity loss, retaining its functionality throughout.
As background, DRAQ7™’s DNA-specificity allows monitoring cell-by-cell while spectral properties permit incorporation in multi-colour experiments e.g. with vital probes, mitochondrial health probes or Annexin V. DRAQ7™ has previously been shown to be truly cell impermeant yet retains DNA binding and far-red fluorescence of the DRAQ chromophore which makes it compatible with HCS imagers, fluorescence microscopes and cytometers. The excitation/emission properties are particularly suited to penetrative imaging of multi-cellular structures and thick ex-plant tissue sections, whilst limiting risks of short wavelength DNA damage.
Histone Deacetylase 6 (HDAC6) As A Therapeutic Target in Chronic Lymphocytic LeukemiaPoster
This study investigates the role of histone deacetylase 6 (HDAC6) in chronic lymphocytic leukemia and establishes it as a novel therapeutic target for the treatment of this disease.READ MORE
Inhibition of The Auto-inflammation Suppressor Protein ISG15 Triggers Preeclampsia by Blocking Trophoblast Migration and InvasionPoster
In summary, ISG15 expression levels are crucial for trophoblast morphology and function (migration/invasion). By blocking trophoblast invasion, reduced ISG15 levels could contribute to impaired spiral artery transformation that reduces utero-placental blood flow in preeclampsia. Thus, agents inducing ISG15 expression are likely to be therapeutic in preeclampsia.
Magnetic-microcontact printing based ECM nano-patterning allows homogeneous controlling of cell growth and behaviorPoster
Magnetic – microcontact printing of extracellular matrix proteins (ECM) results in cell microarrays (CMA) showing homogeneous cell growth and attachment on defined patterns. This greatly reduces bias in the analysis of cell responses to toxins, drugs or other stimuli.READ MORE