PASEF Enhances the Speed, Sensitivity and Selectivity of Label-free Quantification
Poster Apr 05, 2019
The Parallel Accumulation Serial Fragmentation (PASEF) method enables faster acquisition of fragment ion spectra from isolated precursor ions, improving sensitivity without sacrificing spectral quality.
However, the volume of data that is generated and the addition of an extra separation dimension can constitute a challenge for the software.
Download this poster to learn how an adapted version of PEAKS™:
- Enables identifications and label-free quantification from PASEF runs
- Enhances the speed, sensitivity and selectivity of label-free quantification
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.