Quantitative Live-Cell Analysis Using Automated Long-Term Imaging
Poster Sep 13, 2017
Joe Clayton and Peter Banks
Characterizing cell proliferation is a crucial aspect of biological research and therapeutic drug development. Most current cell proliferation assays rely on indirect biochemical metrics that are limited by artifacts or imaging-based endpoint measures.
Here we describe a continuous live-cell assay for determining cell proliferation profiles using the BioSpa Automated Live Cell Imaging System, consisting of BioSpa 8 and Cytation 5. This fully automated method enables quantitative and phenotypic long-term analysis of cell growth using non-invasive measures of confluence or direct cell count. To demonstrate the abilities of this system to conduct robust and reproducible kinetic proliferation assays, NIH3T3, HeLa, and HCT116 cell growth was followed for five days. All three cell types exhibited robust logarithmic growth up to full confluence with doubling times consistent with literature values. Additionally, to demonstrate the ability of this system to screen pharmacological agents, cell proliferation profiles for cells cultured with eight concentrations of two literature-standard inhibitory compounds were generated. Calculated IC50 values were used to measure drug response for each compound and cell type.
A New Method for Analyzing MSe/All Ions Fragmentation in Xenobiotic Metabolism StudiesPoster
During early drug discovery, the study of metabolism plays an essential role in determining which drug candidates move forward into development and later stages. As an alternative to traditional Data Dependent Acquisition (DDA), the use of MSE/All Ions Fragmentation (AIF) has become common in metabolite identification workflows for the analysis of metabolic hot spots. Here we present a solution for analysis of MSE/AlF in metID studies.READ MORE
Extracorporeal shockwave therapy accelerates motor axon regeneration despite a phenotypically mismatched environmentPoster
A femoral nerve defect model was adapted for the evaluation of proregenerative effects of extracorporeal shockwave therapy (ESWT). Functional evaluation, histology and qRT-PCR data show differences between sensory and motor-derived nerve transplants and a pro-regenerative effect of ESWT. These data provide evidence for the clinical application of ESWT after autologous nerve transplantation as a novel non-invasive method.READ MORE