Superporous SIKVAV-modified PHEMA scaffold promotes stem cell adhesion
Poster Sep 18, 2009
Poly(2-hydroxyethyl methacrylate) (PHEMA) is successfully used as a scaffold material for tissue engineering; however, it lacks functional groups that support cell adhesion. The laminin-derived peptide sequence SIKVAV is known to mimic the function of laminin, which is one of the biologically active proteins of the extracellular matrix. In this report, the adhesion of mesenchymal stem cells (MSC) to SIKVAV-modified PHEMA was investigated.
We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.READ MORE