The effect of different magnetic nanoparticle coatings on the efficiency of stem cell labeling
Poster Sep 17, 2009
Rat and human MSCs were labeled with maghemite nanoparticles coated with poly-L-lysine, D-mannose-, or poly-dimethylacrylamide. As a control we used either the commercial contrast agent Endorem or uncoated gama-Fe2O3. The best results were achieved with the PLL coating: 70–80% of cells were labelled. Coated maghemite nanoparticles can be used for tracking grafted cells as well as monitoring their migration, proliferation and differentiation in a host organism.
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We found a distinct subpopulation of Tregs within BMSCs. Tregs and BMSCs in co-culture conferred neuroprotection that varied in a dose-dependent manner. Tregs minimized stem cell production of IL-6, a pro-inflammatory cytokine, and inhibited BMSC secretion of FGF-beta, a cytokine related to BMSC proliferation and differentiation. The ratio of Tregs found natively in BMSCs is optimally adapted to provide the maximum neuroprotective benefit of stem cell treatment after ischemic stroke.
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