Fluorophores, the fluorescent molecules used to stain cells, must be chosen wisely for effective cell imaging.
This poster is the perfect guide for selecting the optimal fluorescent dye for your cell imaging system based on their spectral properties, brightness and photostability.
Download this poster to:
- Ensure compatibility for accurate and reproducible results
- Compare fluorescent dyes appropriate for your application
DAPI
447/60
Texas
Red
628/32
GFP
524/24
GFP Cy5 Cy7
Cy7
794/32
DAPI RFP Texas
Red
RFP
593/40
Cy5
692/40
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen™ EVOS™ cell imaging systems
* Initial brightness: Relative indication (1–5; 1 = dimmest and 5 = brightest) of the brightness of the fluorophore, which is a product of the molar extinction coefficient and quantum yield.
** Photostability: Relative measure (1–5) of the percentage of initial fluorescence intensity remaining following 30 seconds of continuous illumination.
For Research Use Only. Not for use in diagnostic procedures. © 2023 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific
and its subsidiaries unless otherwise specified. Cy is a registered trademark of Cytiva. Hoechst is a trademark of Hoechst GmbH. COL27915 1123
Easily find antibodies for cell imaging at thermofisher.com/antibodies
Initial
brightness*
Photostability
in buffer**
Ex/Em
maxima
Invitrogen™ fluorescent
conjugate or cell dye EVOS emission filter
1 1 343/441 Alexa Fluor 350
3 1 404/455 Alexa Fluor Plus 405
4 2 499/520 Alexa Fluor 488
5 4 493/518 Alexa Fluor Plus 488
3 1 498/517 FITC
4 2 492/519 DyLight 488
5 5 300/525 Qdot 525
4 3 518/543 Alexa Fluor 514
3 2 534/553 Alexa Fluor 532
4 2 561/572 Alexa Fluor 546
3 4 553/568 Alexa Fluor 555
4 5 558/572 Alexa Fluor Plus 555
4 4 552/578 TRITC
4 3 579/603 Alexa Fluor 568
4 4 590/618 Alexa Fluor 594
4 4 590/618 Alexa Fluor Plus 594
4 3 595/613 Texas Red
3 3 631/650 Alexa Fluor 633
3 3 633/648 Alexa Fluor 635
5 3 650/671 Alexa Fluor 647
5 4 658/675 Alexa Fluor Plus 647
5 3 645/664 Cy5
3 2 687/704 Alexa Fluor Plus 680
1 1 696/719 Alexa Fluor 700
3 1 752/776 Alexa Fluor 750
2 2 782/805 Alexa Fluor 790
3 3 789/794 Alexa Fluor Plus 800
5 5 352/455 Hoechst
4 5 359/461 DAPI
3 3 489/508 GFP
3 3 490/512 MitoTracker Green
5 3 505/513 CellMask Green Actin
4 5 545/573 CellMask Orange Actin
3 3 551/575 MitoTracker Orange
4 4 555/583 RFP
4 3 578/598 MitoTracker Red
4 4 587/610 mCherry
3 3 641/662 MitoTracker Deep Red
3 3 653/671 CellMask Deep Red
5 5 662/680 SYTOX Deep Red
|
300
|
331
|
361
|
392
|
423
|
451
|
482
|
512
|
543
|
573
|
604
|
635
|
665
|
696
|
726
|
757
|
788
|
816
|
847
|
877
|
908
|
938
355 405 488 532 561 610 637 808 Wavelength (nm)
