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Reagents for Transfecting Human Embryonic Stem Cells
Product News

Reagents for Transfecting Human Embryonic Stem Cells

Reagents for Transfecting Human Embryonic Stem Cells
Product News

Reagents for Transfecting Human Embryonic Stem Cells


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AMSBIO reports on how it has worked with Singapore General Hospital (Singapore) to help them identify a suitable reagent to transfect their human embryonic stems cells.

Historically the research group of Prof Tan Eng-King had used GeneIN, a transfection reagent specifically developed for stem cells which is now no longer available. Working with AMSBIO, Singapore General Hospital switched to GenePORTER® 2 to transfect their human embryonic stem cells. Senior researcher - Dr Christine Chan Hui Shan commented “Human embryonic stem cells are notoriously difficult-to-transfect primary cells. GenePORTER 2 enabled us to deliver our target genes into hESCs with an improved efficiency, compared to some other commercially available transfection reagents."

GenePORTER® 2 transfection reagent is a proprietary lipid formulation designed to provide high transfection efficiencies in a broad range of mammalian cells. GenePORTER® 2 is designed to be effective in difficult-to-transfect cells, such as PC-12, macrophages, and primary cells. Developed using a high-throughput transfection assay that screens a large library of cationic lipids, the GenePORTER® 2 reagent was selected based on high transfection efficiency, low cytotoxicity, and exceptional stability. Additionally, because of its minimized interaction with serum components, the GenePORTER® 2 transfection reagent can be used for transfection in serum containing media.


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